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- Email: info@nsjbio.com
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Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.
A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.
Aliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
Actin, WB loading control
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