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Home >> Antibodies >> GAPDH Antibody Loading Control

GAPDH Antibody Loading Control [clone GAP259] (N1074)

  Catalog No Formulation Size Price (USD)  
Image N1074-100UG 0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 429
Image
N1074-25UG 0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 25 ug 219
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Western blot testing of human samples with GAPDH antibody at 0.5ug/ml.
Western blot testing of mouse samples with GAPDH antibody at 0.5ug/ml.
Western blot testing of human/mouse samples with GAPDH antibody at 0.5ug/ml.
IHC testing of FFPE human liver tissue with GAPDH antibody at 3ug/ml.
Species Reactivity Human, Mouse, Rat, Primate, Dog
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name GAP259
Purity Protein G Affinity Chromatography
Buffer 1X PBS, pH 7.4
Gene ID 2597
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 3-5ug/ml
Limitations This GAPDH antibody loading control is available for research use only.
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Description

Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

Application Notes

Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.

Immunogen

A recombinant protein fragment from C-terminal GAPDH was used as the immunogen for the GAPDH antibody.

Storage

Aliquot and store the loading control antibody frozen at -20oC or colder to avoid repeated freeze-thaw cycles.

Alternate Names

Glyceraldehyde 3 phosphate dehydrogenase, G3P, G3PD, G3PDH, GAPD

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