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Home >> Antibodies >> Beta Actin Antibody

Beta Actin Antibody [clone 8H10D10] (F40187)

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  Catalog No Formulation Size Price (USD)  
Image F40187-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F40187-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from HepG2, HL-60 cell line (left to right) using beta-Actin antibody at 1:1000 for each lane.
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Western blot analysis of lysate from HepG2, HL-60 cell line (left to right) using beta-Actin antibody at 1:1000 for each lane.
Immunohistochemical analysis of paraffin-embedded human spleen using beta-Actin antibody at 1:25 dilution.
Western blot analysis of lysate from HepG2, HL-60, mouse NIH3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 lysate (left to right) using beta-Actin antibody at 1:1000 for each lane.
Beta-Actin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG
Clone Name 8H10D10
Purity Purified
UniProt P60709
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Limitations This beta-Actin antibody is available for research use only.
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Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus. It is classically referred to as being a 'nonmuscle' actin, however expression is ubiquitous, strongly in lymph tissue and weakly in heart and muscle.

Application Notes

Titration of the beta-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

ACTB recombinant protein was used to produce this monoclonal beta Actin antibody.

Storage

Aliquot the beta-Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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