NSJ Bioreagents

Cytokeratin 7 antibody can be used to identify and study the expression of Cytokeratin 7, a key component of the cytoskeleton in epithelial cells, playing a crucial role in maintaining cell structure and function. This ubiquitous protein has garnered much attention in the field of cell biology due to its involvement in various physiological and pathological processes. Cytokeratins are a family of intermediate filament proteins that are expressed in epithelial tissues. Cytokeratin 7 protein, specifically, is commonly found in glandular epithelia, such as the breast, lung, and reproductive organs. Its expression is typically associated with cells undergoing differentiation or proliferation, making it a valuable marker for identifying specific cell types. Cytokeratin 7 protein helps maintain the structural integrity of epithelial cells, providing support and stability to these tissues. Its expression is essential for normal cell function and contributes to the overall health of the body. Additionally, Cytokeratin 7 protein has been implicated in processes such as cell migration and tissue repair, highlighting its importance in various physiological functions.

Dysregulation of Cytokeratin 7 protein has been linked to several diseases, including cancer. Aberrant expression of this protein has been observed in various cancer types, such as breast cancer, lung cancer, and ovarian cancer. Elevated levels of Cytokeratin 7 protein in tumor cells have been associated with poor prognosis and advanced disease stage, highlighting its potential as a biomarker for cancer diagnosis and prognosis.

Furthermore, Cytokeratin 7 protein has been identified as a target for therapeutic intervention in certain diseases. Research has shown that targeting Cytokeratin 7 protein in cancer cells can inhibit tumor growth and metastasis, offering a promising avenue for the development of novel treatment strategies.

Cytokeratin 7 antibody is a valuable tool for researchers and clinicians in the fields of cell biology, cancer research, and diagnostics. This versatile antibody targets Cytokeratin 7, a protein that plays a key role in maintaining the structural integrity of epithelial cells. Different assays make use of a Cytokeratin 7 antibody, including western blot, immunohistochemistry, flow cytometry, and immunofluorescence, to study this protein.

Western Blot:

One of the most common assays used to detect Cytokeratin 7 expression is western blotting. In this technique, proteins are separated by gel electrophoresis based on their size and charge, then transferred to a membrane for detection with Cytokeratin 7 antibody. Western blotting allows researchers to determine the presence and quantity of Cytokeratin 7 in cell lysates or tissue samples.

Immunohistochemistry:

Immunohistochemistry is another powerful tool for visualizing Cytokeratin 7 in tissue samples. By using a Cytokeratin 7 antibody in conjunction with a chromogen, researchers can localize Cytokeratin 7 within specific cell types or tissues. This technique is widely used in pathology to aid in the diagnosis of various cancers, as Cytokeratin 7 expression is often altered in malignant cells.

Flow Cytometry:

Flow cytometry is a high-throughput technique that allows for the simultaneous analysis of multiple parameters in individual cells. By labeling cells with Cytokeratin 7 antibody conjugated to a fluorophore, researchers can quantify Cytokeratin 7 expression in a cell population. Flow cytometry is especially useful for studying heterogeneous cell populations or rare cell subsets.

Immunofluorescence:

Immunofluorescence is a technique that uses fluorescently labeled antibodies to visualize proteins within cells. By using a Cytokeratin 7 antibody in immunofluorescence, researchers can study the subcellular localization of Cytokeratin 7 and its interactions with other proteins. This technique is invaluable for understanding the function of cytokeratin 7 in cellular processes.

Cytokeratin 7 protein plays a crucial role in maintaining cell structure and function, with implications in both health and disease. Its association with various physiological processes and its dysregulation in disease highlight the importance of further research into this protein. By understanding the role of Cytokeratin 7 protein, we can gain valuable insights into the mechanisms underlying disease progression and potentially develop targeted therapies for various disorders. The Cytokeratin 7 antibody is a versatile tool with a wide range of applications in cell biology, cancer research, and diagnostics. By utilizing assays such as western blot, immunohistochemistry, flow cytometry, and immunofluorescence, researchers can gain valuable insights into the expression and function of Cytokeratin 7 in a variety of experimental settings. Whether studying epithelial cell biology or investigating cancer biomarkers, Cytokeratin 7 antibody is sure to be a useful and valuable reagent.