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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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Recombinant Cytokeratin 7 antibody detects cytokeratin 7, a type II intermediate filament protein encoded by the KRT7 gene. Cytokeratin 7 is expressed in simple epithelia, including those of the lung, breast, ovary, and urinary tract. Because of its tissue distribution, cytokeratin 7 is a widely used marker in cancer pathology, where it helps identify the origin of tumors. Its expression pattern complements that of cytokeratin 20, making the CK7/CK20 profile a key diagnostic tool.
Cytokeratin 7 provides mechanical stability to epithelial cells and contributes to cellular polarity and signaling. It interacts with other cytokeratins to form intermediate filament networks that protect cells from mechanical and chemical stress. Beyond structural roles, cytokeratin 7 participates in intracellular signaling pathways that influence cell growth and differentiation. Dysregulated expression has been observed in carcinomas, where it aids in tumor classification.
The Recombinant Cytokeratin 7 antibody clone rOV-TL12/30 ensures reliable and reproducible detection of CK7. Recombinant manufacturing provides consistent performance across batches, making it dependable for diagnostic and research use. Clone rOV-TL12/30 has been applied in oncology to evaluate carcinomas of the lung, breast, and ovary, in pathology to classify tumors of unknown primary origin, and in developmental biology to study epithelial differentiation.
Research using clone rOV-TL12/30 has clarified how CK7 expression patterns contribute to tumor classification and patient management. For example, CK7 positive and CK20 negative staining is typical of lung and breast carcinomas, while other combinations point to gastrointestinal or urothelial cancers. This diagnostic utility underscores the importance of CK7 detection in pathology.
Beyond diagnostics, clone rOV-TL12/30 supports studies in tissue biology and regenerative research. CK7 expression marks certain progenitor cells and contributes to understanding how epithelia maintain integrity and respond to injury. This antibody continues to support both basic and translational studies across multiple fields.
NSJ Bioreagents provides this Recombinant Cytokeratin 7 antibody to support studies in cancer biology, epithelial development, and diagnostic pathology. Alternate names include KRT7 antibody, CK7 antibody, keratin 7 antibody, and simple epithelium cytoskeletal protein antibody.
The stated application concentrations are suggested starting amounts. Optimal dilution of the recombinant Cytokeratin 7 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the recombinant Cytokeratin 7 antibody.
Store the recombinant Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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