Keratin 7 Antibody SPM270 / Cytokeratin 7 Mouse MAb [clone SPM270] (V2655)

Description

Keratin 7 Antibody SPM270 recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

For an alternative antibody targeting the same protein, see our Cytokeratin 7 Antibody OV-TL12/30 (V2654), a mouse monoclonal antibody validated for WB, IHC, IF, and flow cytometry.

Application Notes

Optimal dilution of the Keratin 7 Antibody SPM270 should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the Keratin 7 Antibody SPM270.

Storage

Store the Keratin 7 Antibody SPM270 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Keratin 7 antibody, CK7 antibody, CK-7 antibody, KRT7 antibody, Cytokeratin-7 antibody, Keratin Type II Cytoskeletal 7 antibody, Type II Cytokeratin 7 antibody, Cytokeratin 7/CK7 antibody, K7 antibody