CD11a Antibody / LFA-1 / ITGAL

The CD11a antibody can be used to study and characterize the CD11a protein. Also known as Lymphocyte function-associated antigen-1 (LFA-1), CD11a is a crucial cell surface protein that plays a vital role in various biological processes. From immune response to cell adhesion, CD11a is important in maintaining cellular functions. CD11a is a type I transmembrane protein that is a component of the integrin alpha-L beta-2 heterodimer, also known as lymphocyte function-associated antigen-1 (LFA-1). It is primarily found on the surface of leukocytes, including T cells, B cells, and natural killer cells. CD11a is involved in cell adhesion, migration, and signaling, making it an essential protein for immune responses. One of the key functions of CD11a is its role in mediating cell adhesion. It interacts with its ligand, intercellular adhesion molecule-1 (ICAM-1), on the surface of other cells, facilitating cell-cell interactions. This interaction is crucial for immune cell migration, extravasation, and immune surveillance. Additionally, CD11a is involved in signal transduction pathways that regulate T cell activation, proliferation, and differentiation. The CD11a protein plays a pivotal role in the immune response, as it is essential for T cell activation and cytotoxicity. Dysfunction of CD11a has been associated with various immunological disorders, including autoimmune diseases, inflammatory conditions, and cancer. Understanding the role of CD11a in these diseases can provide valuable insights into potential therapeutic targets and diagnostic biomarkers. Given its significance in immune responses and cell adhesion, CD11a has potential applications in various fields, including immunology, oncology, and drug development. Researchers are exploring the use of CD11a as a therapeutic target for autoimmune diseases, as well as a biomarker for cancer diagnosis and prognosis. Additionally, CD11a-targeted therapies are being developed for the treatment of inflammatory conditions and infectious diseases.

 

Here we will touch on the diverse applications of CD11a antibody, including flow cytometry, western blot, immunohistochemistry, immunocytochemistry, immunofluorescence, and ELISA.

Flow Cytometry:

CD11a antibody is commonly used in flow cytometry to analyze the expression of CD11a on cell surfaces. This technique allows for the identification and quantification of different cell populations based on their CD11a expression levels.

Western Blot:

By using CD11a antibody in western blotting, researchers can detect and analyze the presence of CD11a in protein samples. This technique is essential for studying the role of CD11a in various cellular processes.

Immunohistochemistry:

Immunohistochemistry enables the visualization of CD11a in tissue samples. Using CD11a antibody in this assay, researchers can investigate the distribution and localization of CD11a within different tissues.

Immunocytochemistry:

Similar to immunohistochemistry, immunocytochemistry utilizes CD11a antibody to visualize the protein within cells. This assay is particularly useful for studying the subcellular localization of CD11a in different cell types.

Immunofluorescence:

Immunofluorescence is a powerful technique that combines the specific binding of CD11a antibody with fluorescent visualization. This assay allows for the precise localization of CD11a within cells, providing valuable insights into its function.

ELISA:

ELISA (enzyme-linked immunosorbent assay) is a sensitive technique used for the quantitative detection of proteins, including CD11a. By using CD11a antibody in ELISA, researchers can accurately measure the levels of CD11a in biological samples.

The CD11a antibody is a versatile tool that can be used in a wide range of assays to study the expression and function of CD11a in different biological contexts. Whether you are interested in flow cytometry, western blot, immunohistochemistry, immunocytochemistry, immunofluorescence, or ELISA, CD11a antibody is sure to provide valuable insights into your research.