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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2581 to 2590
    CD19 Antibody / CD19 molecule (clone CD19/4260R)
    Catalog No : V6034
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Microvalidated
    Recrabbitmono
    Western blot analysis of CD19/CD19 molecule antibody in human tonsil tissue lysate. A strong band is detected at approximately 95 kDa, consistent with the predicted molecular weight of CD19 (Cluster of Differentiation 19), a glycosylated B cell surface receptor. The observed band migrates slightly above the core protein size, which is expected due to N-linked glycosylation of CD19. Blot was probed with CD19/CD19 molecule antibody (clone CD19/4260R).
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    MUC16 Antibody / Cancer antigen 125 (clone CA125/9923R)
    Catalog No : V6041
    Applications : ELISA, IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of CA125 Antibody (Clone CA125/9923R) in FFPE human ovarian carcinoma tissue. Focal membranous and apical cytoplasmic HRP-DAB brown staining is observed in malignant epithelial cells, consistent with MUC16 / ovarian cancer antigen 125 expression in ovarian carcinoma, while surrounding stromal cells are largely negative. This CA125 Antibody for IHC demonstrates distinct epithelial surface localization in tumor cells. The inset shows PBS used in place of the primary antibody as a secondary-only negative control. Heat-induced epitope retrieval was performed in 10mM Tris with 1mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes.
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    MUC16 Antibody / Ovarian cancer antigen 125 (clone CA125/13895R)
    Catalog No : V6042
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of Ovarian cancer antigen 125 Antibody (Clone CA125/13895R) in FFPE human ovarian carcinoma tissue. Prominent membranous and apical cytoplasmic HRP-DAB brown staining is observed in malignant epithelial cells, consistent with MUC16 / CA125 overexpression in ovarian carcinoma, while adjacent stromal elements are largely negative. Clone CA125/13895R demonstrates distinct cell surface localization. The inset shows PBS used in place of the primary antibody as a secondary-only negative control. Heat-induced epitope retrieval was performed in 10mM Tris with 1mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes.
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    CD70 Antibody / CD70 molecule (clone CD70/12768R)
    Catalog No : V6046
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry of CD70 Molecule Antibody in human renal cell carcinoma tissue. FFPE renal cell carcinoma shows strong membranous HRP-DAB brown staining in tumor epithelial cells, consistent with CD70 expression in malignant cells. Clone CD70/12768R was used for detection. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes. Inset shows PBS used in place of primary antibody as a secondary-only negative control.
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    CDKN2A Antibody / p14ARF (clone MSVA-016R)
    Catalog No : V6059
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    p16 INK4a/CDKN2A antibody (clone MSVA-016R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas. <BR>Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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    Estrogen Receptor alpha Antibody (clone MSVA-564R)
    Catalog No : V6071
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry tissue microarray analysis of Estrogen receptor alpha expression. This recombinant Estrogen receptor alpha/ER alpha antibody (clone MSVA-564R) was evaluated by immunohistochemistry on formalin-fixed, paraffin-embedded human tissue microarrays covering a broad range of normal and cancer tissues. Staining demonstrates predominantly nuclear immunoreactivity in hormone-responsive epithelial tissues consistent with known Estrogen receptor alpha / ER alpha / ESR1 expression patterns, with limited staining in tissues lacking estrogen signaling activity. Cancer tissues show variable nuclear positivity depending on tumor type and hormone responsiveness. Overall staining distribution and relative expression patterns are concordant with publicly available expression data reported by the Human Protein Atlas, supporting the biological relevance of the observed immunoreactivity.
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    PMEL17 / Melanoma gp100 Antibody (clone MSVA-617R)
    Catalog No : V6111
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Immunohistochemistry analysis of gp100 Melanoma Marker / PMEL antibody (clone MSVA-617R) in human normal and cancer tissue microarrays. FFPE tissue microarrays demonstrate strong HRP-DAB brown cytoplasmic staining in malignant melanoma samples, while the majority of non-melanocytic normal tissues and other tumor types show minimal to no specific staining. In normal skin, melanocytes exhibit expected positive staining, consistent with melanocytic lineage-restricted expression of PMEL. The observed staining pattern across normal and cancer tissues aligns with expression data reported in the Human Protein Atlas.
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    p53 Antibody / TP53 (clone MSVA-053R)
    Catalog No : V6124
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of p53 IHC antibody in human normal and cancer tissue microarrays (clone MSVA-053R). FFPE tissue microarrays demonstrate predominantly nuclear HRP-DAB brown staining in selected tumor types, consistent with accumulation of Tumor protein 53 in neoplastic cells, while most normal tissues show minimal to weak nuclear signal under physiologic conditions. Strong nuclear staining is observed in subsets of colorectal, breast, ovarian, and other carcinomas, whereas many non-neoplastic tissues display low basal expression. The overall staining distribution across normal and malignant tissues aligns with established TP53 expression patterns and publicly available data from the Human Protein Atlas.
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    PAX8 Antibody / Paired box gene 8 (clone MSVA-708R)
    Catalog No : V6133
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of PAX8 antibody in human tissue microarrays. Formalin-fixed, paraffin-embedded normal and cancer tissue microarrays were stained with Paired box protein Pax-8 rabbit recombinant monoclonal antibody (clone MSVA-708R). Nuclear HRP-DAB brown staining is observed in expected PAX8-positive tissues, including renal tubular epithelium, thyroid follicular cells, and Mullerian-derived epithelium, while most non-lineage tissues show minimal to no nuclear signal. In cancer tissues, strong nuclear staining is present in renal cell carcinoma, thyroid carcinoma, and Mullerian-derived tumors, consistent with known lineage-specific expression patterns. The staining distribution across normal and malignant samples aligns with reported expression profiles in the Human Protein Atlas. Negative tissues demonstrate absence of specific nuclear staining, supporting specificity of the antibody.
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    CD7 Antibody / T cell antigen CD7 (clone MSVA-007R)
    Catalog No : V6142
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of CD7 Antibody for IHC in human tissue microarrays. FFPE normal and cancer tissues demonstrate strong membranous HRP-DAB brown staining in T cell-rich compartments including thymus, tonsil, spleen, and lymph node, consistent with CD7-positive lymphocytes. Most non-lymphoid tissues such as liver, kidney, pancreas, and smooth muscle show minimal to absent staining, reflecting the lineage-restricted expression pattern of CD7. In cancer panels, robust staining is observed in T cell-derived lymphomas, while the majority of non-hematologic tumors exhibit little to no specific signal. These staining patterns are consistent with publicly available expression data reported in the Human Protein Atlas. Clone MSVA-007R is a recombinant rabbit monoclonal antibody engineered for reliable detection of CD7 in formalin-fixed, paraffin-embedded human tissues.
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