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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2581 to 2590
    TG Antibody / Thyroglobulin (clone TGB/4582R)
    Catalog No : V6011
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of Thyroid Globulin / TG antibody in human thyroid tissue (clone TGB/4582R). FFPE human thyroid sections demonstrate strong HRP-DAB brown cytoplasmic staining in follicular epithelial cells, with intense brown chromogenic signal filling the follicular lumina corresponding to colloid material. The staining pattern is consistent with abundant Thyroglobulin synthesis and secretion by differentiated thyroid follicular cells. Surrounding stromal elements show minimal staining. The inset shows PBS used in place of primary antibody as a secondary antibody negative control, confirming absence of specific brown chromogenic signal. Heat induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, by heating tissue sections at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.
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    UPK2 Antibody / Uroplakin 2 (clone UPK2/13669R)
    Catalog No : V6017
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of UPK2 antibody in human bladder carcinoma. Formalin-fixed, paraffin-embedded human bladder cancer tissue was stained with Uroplakin II recombinant rabbit monoclonal antibody (clone UPK2/13669R). Strong HRP-DAB brown membranous and cytoplasmic staining is observed in tumor epithelial cells, consistent with Uroplakin 2 expression in urothelial-derived carcinoma. The staining highlights cell membranes and apical surfaces with diffuse cytoplasmic signal in positive tumor cells, while adjacent stromal elements show minimal background staining. Inset: PBS was used in place of the primary antibody as a negative control. Heat-induced epitope retrieval was performed in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95oC followed by cooling at room temperature for 20 minutes.
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    UPK2 Antibody / Uroplakin 2 (clone UPK2/13348R)
    Catalog No : V6018
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of UPK2 antibody in human bladder carcinoma. Formalin-fixed, paraffin-embedded human bladder cancer tissue was stained with Uroplakin II recombinant rabbit monoclonal antibody (clone UPK2/13348R). Strong HRP-DAB brown membranous and cytoplasmic staining is observed in tumor epithelial cells, consistent with Uroplakin 2 expression in urothelial-derived carcinoma. The staining highlights cell membranes with circumferential membranous accentuation and granular cytoplasmic signal, while adjacent stromal tissue shows minimal background staining. Inset: PBS was used in place of the primary antibody as a negative control. Heat-induced epitope retrieval was performed in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95oC followed by cooling at room temperature for 20 minutes.
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    NECTIN4 Antibody / Poliovirus receptor related 4 (clone NECTIN4/13438R)
    Catalog No : V6026
    Applications : FACS, IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of Poliovirus receptor related 4 / NECTIN4 antibody (clone NECTIN4/13438R) in human placental tissue. Formalin-fixed, paraffin-embedded placenta demonstrates membranous HRP-DAB brown staining outlining trophoblastic cells and chorionic villi, consistent with cell surface localization of Nectin-4. Background staining is minimal, and hematoxylin counterstain highlights tissue morphology. The inset shows PBS used in place of primary antibody as a negative control, confirming absence of non-specific secondary antibody binding. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.
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    CD2 Antibody / CD2 molecule (clone LFA2/13325R)
    Catalog No : V6030
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot analysis of CD2 antibody (clone LFA2/13325R) in human MOLT-4 cell lysate. A strong band is observed at approximately 50-55 kDa, consistent with the predicted molecular weight of CD2. The band appears relatively thick and intense, which is expected due to the high endogenous expression of CD2 in T lymphoblastic cells such as MOLT-4, as well as potential heterogeneous glycosylation that can broaden migration on SDS-PAGE. Molecular weight markers are indicated in kDa on the left.
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    CD7 Antibody / CD7 molecule (clone CD7/13034R)
    Catalog No : V6032
    Applications : ELISA
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    SDS-PAGE Analysis of Purified CD7 molecule antibody (clone CD7/13034R). Confirmation of Purity and Integrity of Antibody.
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    CD19 Antibody / CD19 molecule (clone CD19/4260R)
    Catalog No : V6034
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Microvalidated
    Recrabbitmono
    Western blot analysis of CD19/CD19 molecule antibody in human tonsil tissue lysate. A strong band is detected at approximately 95 kDa, consistent with the predicted molecular weight of CD19 (Cluster of Differentiation 19), a glycosylated B cell surface receptor. The observed band migrates slightly above the core protein size, which is expected due to N-linked glycosylation of CD19. Blot was probed with CD19/CD19 molecule antibody (clone CD19/4260R).
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    MUC16 Antibody / Cancer antigen 125 (clone CA125/9923R)
    Catalog No : V6041
    Applications : ELISA, IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of CA125 Antibody (Clone CA125/9923R) in FFPE human ovarian carcinoma tissue. Focal membranous and apical cytoplasmic HRP-DAB brown staining is observed in malignant epithelial cells, consistent with MUC16 / ovarian cancer antigen 125 expression in ovarian carcinoma, while surrounding stromal cells are largely negative. This CA125 Antibody for IHC demonstrates distinct epithelial surface localization in tumor cells. The inset shows PBS used in place of the primary antibody as a secondary-only negative control. Heat-induced epitope retrieval was performed in 10mM Tris with 1mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes.
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    MUC16 Antibody / Ovarian cancer antigen 125 (clone CA125/13895R)
    Catalog No : V6042
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of Ovarian cancer antigen 125 Antibody (Clone CA125/13895R) in FFPE human ovarian carcinoma tissue. Prominent membranous and apical cytoplasmic HRP-DAB brown staining is observed in malignant epithelial cells, consistent with MUC16 / CA125 overexpression in ovarian carcinoma, while adjacent stromal elements are largely negative. Clone CA125/13895R demonstrates distinct cell surface localization. The inset shows PBS used in place of the primary antibody as a secondary-only negative control. Heat-induced epitope retrieval was performed in 10mM Tris with 1mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes.
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    CD70 Antibody / CD70 molecule (clone CD70/12768R)
    Catalog No : V6046
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry of CD70 Molecule Antibody in human renal cell carcinoma tissue. FFPE renal cell carcinoma shows strong membranous HRP-DAB brown staining in tumor epithelial cells, consistent with CD70 expression in malignant cells. Clone CD70/12768R was used for detection. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes. Inset shows PBS used in place of primary antibody as a secondary-only negative control.
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