Calcium-Binding Protein PV Antibody / PVALB [clone PVALB/12794R] (V5983)

Western blot analysis of Calcium-Binding Protein PV/PVALB antibody (clone PVALB/12794R) in brain tissue lysates from multiple species. Brain lysates from human, mouse, and rat show clear immunoreactive bands at approximately 12 kDa, consistent with the predicted molecular weight of Parvalbumin based on its amino acid sequence. No specific band is observed in hamster brain, guinea pig brain, or rabbit brain under the conditions tested. The detected bands migrate in the expected low molecular weight range for this small cytosolic EF-hand calcium-binding protein, supporting species-reactive detection of Parvalbumin in human, mouse, and rat brain tissue.

Analysis of Protein Array containing more than 19,000 full-length human proteins using Calcium-Binding Protein PV/PVALB antibody (clone PVALB/12794R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Western blot analysis of Calcium-Binding Protein PV/PVALB antibody (clone PVALB/12794R) in human brain tissue lysate. A distinct immunoreactive band is observed at approximately 12 kDa, consistent with the predicted molecular weight of Parvalbumin based on its amino acid sequence. The band migrates in the expected low molecular weight range for this small cytosolic EF-hand calcium-binding protein. The signal intensity supports detection of Parvalbumin in human brain tissue under the conditions tested.

Immunohistochemistry analysis of Calcium-Binding Protein PV/PVALB antibody (clone PVALB/12794R) in human tonsil tissue. FFPE human tonsil demonstrates cytoplasmic HRP-DAB brown staining in scattered cells within the lymphoid tissue, consistent with Parvalbumin expression. Positive cells are distributed throughout the tissue and exhibit granular cytoplasmic staining, while surrounding lymphocytes show minimal to no specific signal. The inset represents a secondary antibody negative control in which PBS was used in place of the primary antibody and shows absence of specific staining. Heat induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes prior to immunostaining.