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Home >> Antibodies >> Signal Transducer 6 Antibody / STAT6 Recombinant Monoclonal Antibody

Signal Transducer 6 Antibody / STAT6 Recombinant Monoclonal Antibody [clone STAT6/12798R] (V6003)

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  Catalog No Formulation Size Price (USD)  
Image V6003-100UG 0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 559
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V6003-20UG 0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 259
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V6003SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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Signal Transducer 6 Antibody WB Validation in HeLa WT and STAT6 Knockdown Cells. Western blot analysis of human HeLa wild-type (WT, lane 1) and STAT6 shRNA knockdown (KD, lane 2) cell lysates using STAT6 Recombinant Monoclonal Antibody clone STAT6/12798R. A band near 90-100 kDa is detected in WT cells and is markedly reduced in STAT6 knockdown cells, supporting target-dependent recognition. Hsp90 alpha serves as the loading control.
Signal Transducer 6 Antibody WB Analysis of Human, Mouse and Rat Skin Tissue. Western blot analysis of human, mouse, and rat skin tissue lysates using STAT6 Recombinant Monoclonal Antibody clone STAT6/12798R. A prominent band near 90-100 kDa is detected in the human sample, consistent with the expected molecular weight of STAT6. No corresponding prominent band is evident in the mouse or rat samples under the conditions tested.
Signal Transducer 6 Antibody WB Analysis of Human Kidney Tissue. Western blot analysis of human kidney tissue lysate using STAT6 Recombinant Monoclonal Antibody clone STAT6/12798R. A prominent band is detected at approximately 90-100 kDa, consistent with the expected molecular weight of STAT6.
Signal Transducer 6 Antibody IHC Staining of Human Liposarcoma Tissue. Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liposarcoma tissue using STAT6 Recombinant Monoclonal Antibody clone STAT6/12798R. Brown DAB staining is observed in numerous spindle-shaped tumor cells, including nuclear staining. Heat-induced epitope retrieval was performed using Tris-EDTA buffer (pH 9), followed by hematoxylin counterstaining.
Signal Transducer 6 Antibody SDS-PAGE (Reducing vs Non-Reducing). SDS-PAGE analysis of Signal Transducer 6 antibody clone STAT6/12798R under reducing (R) and non-reducing (NR) conditions, demonstrating expected antibody heavy and light chain band patterns.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name STAT6/12798R
UniProt P42226
Localization Cytoplasm, Nucleus
Applications Immunohistochemistry (FFPE): 1-2ug/ml
Knockdown
Western Blot: 2-4ug/ml
Limitations This Signal Transducer 6 Antibody / STAT6 Recombinant Monoclonal Antibody is available for research use only.
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Description

Signal Transducer 6 Antibody / STAT6 Recombinant Monoclonal Antibody targets Signal Transducer and Activator of Transcription 6 (STAT6), a transcription factor that mediates cellular responses to interleukin-4 (IL-4) and interleukin-13 (IL-13). STAT6 belongs to the STAT family of signaling proteins, which transmit information from activated cytokine receptors to the nucleus. Its regulatory functions influence immune cell differentiation, inflammatory responses, and transcriptional programs involved in tissue homeostasis. STAT6 is particularly important in type 2 immunity, where it coordinates gene expression associated with allergic inflammation and responses to parasitic infection.

STAT6 activation begins when IL-4 or IL-13 binds its corresponding receptor complex, triggering receptor-associated Janus kinases (JAKs). These kinases phosphorylate STAT6 at tyrosine 641, promoting dimerization and nuclear accumulation. Activated STAT6 dimers recognize specific DNA sequences and regulate cytokine-responsive genes. The pathway is controlled by phosphatases, inhibitory signaling proteins, and mechanisms that regulate STAT6 localization and transcriptional activity. Because STAT6 can be present in both cytoplasmic and nuclear compartments, its abundance and localization must be distinguished from phosphorylation-dependent activation.

In adaptive immunity, STAT6 promotes differentiation of naive CD4-positive T cells toward the T helper 2 (Th2) lineage. It contributes to expression of GATA3 and other regulators of type 2 immune responses. STAT6 also participates in IL-4-dependent immunoglobulin class switching and influences B-cell activation. In macrophages, IL-4 and IL-13 signaling through STAT6 supports alternative activation programs associated with tissue repair, extracellular matrix remodeling, and immune regulation. These activities connect STAT6 with interactions between immune cells and their surrounding tissue environments.

Altered STAT6 signaling is implicated in asthma, atopic disease, chronic inflammation, and cancer-associated immune responses. In certain tumors, STAT6 expression and localization may also be influenced by molecular abnormalities unrelated to conventional cytokine stimulation. For example, nuclear STAT6 immunoreactivity is an established characteristic of many solitary fibrous tumors associated with NAB2-STAT6 gene fusions. Consequently, nuclear staining should be interpreted within the appropriate molecular and histopathological context rather than treated as definitive evidence of active IL-4 or IL-13 signaling.

Signal Transducer 6 Antibody supports investigations of cytokine-responsive transcription, immune differentiation, and disease-associated changes in STAT6 expression. Understanding how STAT6 abundance, phosphorylation, and subcellular localization are regulated remains important for distinguishing normal immune signaling from persistent or dysregulated transcriptional responses.

For additional STAT6 research, explore our STAT6 Antibody, featuring knockout-validated Western blot data and supporting immunohistochemistry, immunofluorescence, and flow cytometry results.

Application Notes

Optimal dilution of the Signal Transducer 6 Antibody / STAT6 Recombinant Monoclonal Antibody should be determined by the researcher.

Immunogen

A recombinant fragment (around amino acids 600-801) of human STAT6 protein (exact sequence is proprietary) was used as the immunogen for the Signal Transducer 6/STAT6 antibody.

Storage

Signal Transducer 6/STAT6 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

Alternate Names

STAT6 antibody, Signal transducer and activator of transcription 6 antibody, STAT6 signaling antibody, STAT6 nuclear transcription factor antibody, STAT6 knockdown antibody

Related Products

   1.   View all STAT6/Signal transducer and activator of transcription 6 antibodies

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