Cytotoxic Lymphocyte Perforin Antibody / PRF1 [clone PRF1/9681R] (V5975)

Western blot analysis of Cytotoxic Lymphocyte Perforin Antibody in HDLM-2 cell lysate. HDLM-2 lysate shows a prominent band at approximately 70 kDa, consistent with the predicted molecular weight of Perforin-1. The signal appears as a strong, specific band under reducing SDS-PAGE conditions, with minor higher molecular weight signal that may represent protein aggregation or incompletely reduced species. The observed band aligns with endogenous PRF1 expression, which is expected in activated or cytotoxic lymphoid cell populations.

Immunohistochemistry analysis of Cytotoxic Lymphocyte Perforin Antibody in human spleen tissue. Formalin-fixed, paraffin-embedded human spleen stained with Perforin-1 Recombinant Rabbit Monoclonal Antibody (clone PRF1/9681R) demonstrates punctate cytoplasmic HRP-DAB brown staining in scattered lymphoid cells within the white pulp, consistent with perforin localization in cytotoxic T lymphocytes and natural killer cells. The majority of surrounding lymphocytes are negative, highlighting the restricted expression pattern of PRF1 in activated cytotoxic subsets. The inset image shows the negative control in which PBS was used in place of the primary antibody, confirming absence of non-specific secondary antibody staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 10mM Tris with 1mM EDTA for 45 minutes at 95oC followed by cooling prior to immunostaining.

SDS-PAGE Analysis of Purified Cytotoxic Lymphocyte Perforin/PRF1 antibody (PRF1/9681R). Confirmation of Purity and Integrity of Antibody.