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Recombinant Mouse Antibodies

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Recombinant Mouse Antibodies

Found all antibodies, displaying 581 to 590
MCM5 Antibody / DNA replication licensing factor MCM5 (clone rMCM5/13646)
Catalog No : V5944
Applications : IHC, WB
Reactivity : Human
Format : Purified
Recmousemono
IHC analysis of formalin-fixed, paraffin-embedded human colon tissue using recombinant Minichromosome maintenance protein 5/MCM5 antibody (recombinant mouse monoclonal clone rMCM5/13646). Strong nuclear brown chromogenic staining is observed in proliferating colonic epithelial cells, consistent with nuclear localization of Minichromosome maintenance protein 5, while surrounding non-proliferative cells show reduced staining. Inset: PBS used in place of primary antibody as secondary-only negative control. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes.
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MMP9 Antibody / Matrix metalloproteinase 9 (clone r15W2)
Catalog No : V5949
Applications : IHC
Reactivity : Human
Format : Purified
Recmousemono
Immunohistochemistry analysis of Gelatinase B / MMP9 antibody (clone r15W2) in human spleen tissue. Formalin-fixed, paraffin-embedded human spleen section shows cytoplasmic brown chromogenic staining in scattered immune cells within the splenic parenchyma, consistent with MMP9 expression, while surrounding lymphoid cells display minimal staining and nuclei appear blue. The inset shows PBS used in place of primary antibody as a negative control with no specific staining observed. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.
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MUC5AC Antibody for IHC / Mucin 5AC (clone MSVA-109M)
Catalog No : V5953
Applications : IHC
Reactivity : Human
Format : Purified
Recmousemono
MUC5AC Antibody for IHC Tissue Microarray (TMA). Immunohistochemistry analysis of Mucin 5AC MUC5AC in formalin-fixed paraffin-embedded human normal and cancer tissue microarrays using recombinant mouse monoclonal MUC5AC antibody clone MSVA-109M. Tissue microarray (TMA) staining with HRP-DAB brown chromogen demonstrates prominent cytoplasmic localization in gastric foveolar epithelium and mucus-secreting epithelial cells, consistent with gastric-type mucin expression, while non-mucinous epithelial tissues and non-epithelial compartments including stromal and mesenchymal tissues show minimal to absent staining. Within tumor tissue microarrays, strong cytoplasmic staining is observed in mucinous and glandular carcinomas, supporting identification of mucin-producing tumor cells and assessment of epithelial differentiation. Evaluation across large TMA panels enables direct comparison of MUC5AC expression across diverse tissue types under standardized conditions. The observed staining patterns align with reported MUC5AC expression profiles in publicly available datasets including the Human Protein Atlas.
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SERPINE1 Antibody / Plasminogen activator inhibitor 1 / PA1-1 (clone rTJA6)
Catalog No : V5962
Applications : IHC, WB
Reactivity : Human
Format : Purified
Recmousemono
Immunohistochemistry analysis of SERPINE1 / PAI-1 antibody in human testis. Formalin-fixed, paraffin-embedded human testis tissue was stained with SERPINE1 / PAI-1 antibody (clone rTJA6). HRP-DAB brown chromogenic signal is observed predominantly in the cytoplasm of seminiferous tubule-associated cells, with staining localized to germ cells and surrounding supporting cells, while nuclei are counterstained blue. The staining pattern is consistent with cytoplasmic localization of Serpin family E member 1 in testicular tissue. The inset image shows PBS used instead of primary antibody, demonstrating absence of specific HRP-DAB brown staining and confirming staining specificity. Heat-induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to antibody incubation.
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PAX2 Antibody / Paired box protein Pax-2 (clone rPAX2/8506)
Catalog No : V5963
Applications : IHC
Reactivity : Human
Format : Purified
Recmousemono
Immunohistochemistry analysis of Paired box protein 2 / PAX2 antibody in human endometrium. FFPE human endometrium tissue was stained with Paired box protein 2 / PAX2 antibody (clone rPAX2/8506). HRP-DAB brown chromogenic signal is observed predominantly in the nuclei of glandular epithelial cells lining the endometrial glands, consistent with the expected nuclear localization of PAX2. Stromal cells show minimal to no specific staining. Nuclei are counterstained blue. The inset image shows PBS used instead of primary antibody, demonstrating absence of specific HRP-DAB brown staining and confirming staining specificity. Heat-induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to antibody incubation.
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CD31 Antibody for IHC / PECAM1 (clone MSVA-031M)
Catalog No : V5967
Applications : IHC
Reactivity : Human
Format : Purified
Recmousemono
CD31 Antibody for IHC Tissue Microarray (TMA). Immunohistochemistry analysis of Platelet endothelial cell adhesion molecule 1 PECAM1, also known as CD31, in formalin-fixed paraffin-embedded human normal and cancer tissue microarrays using recombinant mouse monoclonal CD31 antibody clone MSVA-031M. Tissue microarray (TMA) staining with HRP-DAB brown chromogen demonstrates distinct membranous localization in vascular endothelial cells across multiple tissues, clearly outlining blood vessel linings and capillary networks, while non-endothelial epithelial and stromal compartments show minimal to absent staining. Within tumor tissue microarrays, CD31 highlights tumor-associated vasculature, revealing variable microvessel density and complex vascular architecture within tumor stroma. Evaluation across large TMA panels enables direct comparison of PECAM1 expression across diverse tissue types under standardized conditions. The observed staining patterns align with reported PECAM1 expression profiles in publicly available datasets including the Human Protein Atlas, supporting its use for endothelial visualization and vascular analysis.
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Perforin Antibody / PRF1 (clone rPRF1/8058)
Catalog No : V4101
Applications : WB, IHC-P
Reactivity : Human
Format : Purified
Recmousemono
Western blot testing of human HDLM-2 cell lysate with Perforin-1 antibody (clone rPRF1/8058). Predicted molecular weight ~61 kDa.
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PTGS2 Antibody / Prostaglandin-endoperoxide synthase 2 (clone r4H12)
Catalog No : V5977
Applications : IHC
Reactivity : Human
Format : Purified
Recmousemono
Western blot analysis of NIH3T3 mouse fibroblast cell lysate using PTGS2 / Prostaglandin-Endoperoxide Synthase 2 antibody (clone r4H12). A distinct band is observed at approximately 72-74 kDa, consistent with the predicted molecular weight of PTGS2. The detected signal aligns with the expected size of the inducible Cyclooxygenase 2 protein.
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CD45RA Antibody for IHC / Leukocyte marker (clone MSVA-472M)
Catalog No : V5979
Applications : IHC
Reactivity : Human
Format : Purified
Recmousemono
CD45RA Antibody for IHC Tissue Microarray (TMA). Immunohistochemistry analysis of Protein tyrosine phosphatase receptor type C PTPRC, also known as CD45RA, in formalin-fixed paraffin-embedded human normal and cancer tissue microarrays using recombinant mouse monoclonal CD45RA antibody clone MSVA-472M. Tissue microarray (TMA) staining with HRP-DAB brown chromogen demonstrates strong membranous localization in lymphoid cell populations, prominently highlighting lymphocytes within tonsil, spleen, thymus, and lymph node, while non-hematopoietic epithelial and stromal tissues remain largely negative. Within tumor tissue microarrays, diffuse membranous positivity is observed in CD45RA-positive lymphoid malignancies, while most non-lymphoid tumors remain negative with staining restricted to infiltrating immune cells. Evaluation across large TMA panels enables direct comparison of PTPRC expression across diverse tissue types under standardized conditions. The observed staining patterns align with reported PTPRC expression profiles in publicly available datasets including the Human Protein Atlas, supporting its use for lymphoid cell identification and immune profiling.
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PVALB Antibody / Parvalbumin (clone r2E11)
Catalog No : V5981
Applications : IHC, WB
Reactivity : Human
Format : Purified
Recmousemono
Western blot analysis of Parvalbumin / PVALB antibody (clone r2E11) in human kidney tissue lysate. A distinct immunoreactive band is observed at approximately 12 kDa, consistent with the predicted molecular weight of Parvalbumin based on its amino acid sequence. The detected band migrates in the low molecular weight range as expected for this small cytosolic EF-hand calcium-binding protein. The observed size aligns with the predicted molecular weight, with no prominent higher molecular weight non-specific bands detected under the conditions tested.
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