Recombinant CD20 Antibody [clone rIGEL/773] (V3727)

Description

Recombinant CD20 antibody is designed to detect CD20, a membrane-embedded phosphoprotein encoded by the MS4A1 gene. CD20 is expressed on the surface of pre-B and mature B lymphocytes but absent from stem cells and plasma cells. Its restricted expression and stable surface presence have made CD20 a defining marker for B cells and a central therapeutic target in immunology and oncology. Because of these characteristics, CD20 is extensively studied in immune development, autoimmune disease, and hematologic malignancies.

CD20 has a small extracellular loop and functions as a calcium channel or regulator of calcium flux, contributing to B cell activation and proliferation. While its precise physiological role is not completely defined, its consistent expression pattern across the B cell lineage makes it indispensable in pathology. Alterations in CD20 expression influence immune regulation and have direct clinical implications for therapy.

The Recombinant CD20 antibody clone rIGEL/773 ensures reliable and reproducible recognition of this B cell marker. Recombinant production guarantees uniform performance across experiments, eliminating lot-to-lot variation. Clone rIGEL/773 has been employed in studies of B cell development, autoimmunity, and lymphomas. Peer-reviewed research has reported use of this clone in evaluating CD20 expression in B cell leukemias and lymphomas, highlighting its value for both basic and clinical science.

Research using clone rIGEL/773 has provided insights into how CD20 expression correlates with disease subtype and therapeutic responses. Because CD20 is the target of widely used monoclonal antibody therapies, reliable detection of this antigen is crucial for diagnosis and monitoring. This antibody continues to support investigations into immune regulation, cancer biology, and translational applications that explore new CD20-targeted therapies.

NSJ Bioreagents offers this Recombinant CD20 antibody to support high-quality studies of immune biology, hematology, and therapeutic development. The protein is also known as MS4A1 antibody, B1 antigen antibody, B lymphocyte antigen antibody, and CD20 molecule antibody, illustrating its varied nomenclature across scientific literature.

Application Notes

Titering of the recombinant CD20 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human CD20 protein was used as the immunogen for this recombinant CD20 antibody.

Storage

Recombinant CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.