SERPINE1 Antibody / Serpin family E member 1 / PAI-1 [clone rTJA6] (V5962)

Immunohistochemistry analysis of SERPINE1 / PAI-1 antibody in human testis. Formalin-fixed, paraffin-embedded human testis tissue was stained with SERPINE1 / PAI-1 antibody (clone rTJA6). HRP-DAB brown chromogenic signal is observed predominantly in the cytoplasm of seminiferous tubule-associated cells, with staining localized to germ cells and surrounding supporting cells, while nuclei are counterstained blue. The staining pattern is consistent with cytoplasmic localization of Serpin family E member 1 in testicular tissue. The inset image shows PBS used instead of primary antibody, demonstrating absence of specific HRP-DAB brown staining and confirming staining specificity. Heat-induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to antibody incubation.

Application of SERPINE1 / PAI-1 antibody in western blot. Western blot analysis of SERPINE1 / PAI-1 antibody (clone rTJA6) was performed using human A-431 whole cell lysate. A strong immunoreactive band is observed at approximately 45 kDa, consistent with the predicted molecular weight of Serpin family E member 1. The detected signal aligns with the predicted molecular weight under reducing conditions. PAI-1 is a secreted serpin that can exist in active and latent conformations, and the slightly broadened appearance of the band likely reflects conformational states or differences in glycosylation rather than non-specific binding. The blot demonstrates specific detection of endogenous SERPINE1 in human A-431 cells at its predicted molecular weight.

SDS-PAGE Analysis of Purified SERPINE1/Serpin family E member 1 antibody (rTJA6). Confirmation of Purity and Integrity of Antibody.