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banner Search results for integrin alpha

Itga4 Antibody / Integrin alpha 4

Catalog No : FY12338
Applications : WB, FACS, ELISA
Reactivity : Mouse, Rat
Format : Lyophilized
Flow Cytometry analysis of NIH/3T3 cells using anti-Integrin Itga4 antibody. Overlay histogram showing NIH/3T3 cells stained with (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin Itga4 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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ITGA4 Antibody / Integrin Alpha 4

Catalog No : FY12794
Applications : WB, ELISA
Reactivity : Human
Format : Lyophilized
Western blot analysis of Integrin Alpha 4/ITGA4 using anti-ITGA4 antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA4 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A broad band is detected at ~150 kDa in most samples, consistent with the mature glycosylated alpha 4 subunit; U87 shows a prominent ~130-140 kDa band with a minor lower species, consistent with cell-type-dependent glycosylation and limited processing relative to the ~115 kDa core mass.
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ITGA6 Antibody / Integrin alpha 6 (clone 129CT49.6.5)

Catalog No : F40215
Applications : IF, FACS, IHC, WB, ELISA
Reactivity : Human
Format : Purified
ITGA6 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma.
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ITGA6 Antibody / Integrin alpha 6

Catalog No : RQ5304
Applications : WB, FACS, Direct ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Flow cytometry testing of fixed and permeabilized human HepG2 cells with Integrin alpha 6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Integrin alpha 6 antibody.
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ITGA8 Antibody / Integrin alpha 8

Catalog No : RQ7713
Applications : WB, IF, FACS, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human A431 cells with Integrin alpha 8 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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ITGA9 Antibody / Integrin alpha 9

Catalog No : FY12686
Applications : WB, FACS, ELISA
Reactivity : Human
Format : Lyophilized
Flow Cytometry analysis of SH-SY5Y cells using anti-ITGA9 antibody. Overlay histogram showing SH-SY5Y cells stained with (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ITGA9 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Integrin alpha 4 Antibody / ITGA4 (clone RM268)

Catalog No : R20285
Applications : WB, IHC
Reactivity : Human
Format : Purified
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Western blot testing of human Jurkat cell lysate with recombinant ITGA4 antibody at 1:1000. Predicted molecular weight ~115 kDa, routinely observed at ~150 kDa.
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Integrin alpha E Antibody / CD103 (clone ITGAE/3904R)

Catalog No : V8419
Applications : IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human tonsil with recombinant Integrin alpha E antibody (clone ITGAE/3904R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Integrin alpha E Antibody / CD103 (clone rITGAE/2063)

Catalog No : V8433
Applications : IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human tonsil with recombinant ITGAE antibody (clone rITGAE/2063). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Integrin alpha E Antibody / CD103 (clone ITGAE/2063)

Catalog No : V3848
Applications : IHC-P
Reactivity : Human
Format : Purified
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IHC testing of FFPE human tonsil with CD103 antibody (clone ITGAE/2063). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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