Integrin Alpha E Antibody rITGAE/2063 / Recombinant CD103 Antibody [clone rITGAE/2063] (V8433)

Integrin Alpha E Antibody rITGAE/2063 Staining in Human Tonsil by IHC. Immunohistochemistry of FFPE human tonsil tissue using Integrin Alpha E Antibody (clone rITGAE/2063) shows distinct brown staining in scattered lymphoid cells distributed throughout the tissue. Positive cells are interspersed among a dense population of hematoxylin-counterstained lymphocytes, producing a selective and heterogeneous cellular pattern consistent with CD103 expression in a subset of tonsillar lymphocytes. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 10mM Tris with 1mM EDTA for 20 min followed by cooling before testing. This pattern demonstrates CD103 detection using this Recombinant CD103 Antibody.

Integrin Alpha E Antibody rITGAE/2063 Purity and Integrity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Integrin Alpha E Antibody (clone rITGAE/2063) with 2 ug loaded per lane shows the expected immunoglobulin migration profiles under non-reducing (NR) and reducing (R) conditions. The non-reduced antibody migrates predominantly as an intact species near 150 kDa, while reduction produces prominent heavy and light chain bands at approximately 50 kDa and 25 kDa, respectively. Minimal additional staining is visible, supporting the integrity and purity of this Recombinant CD103 Antibody preparation.