ITGAE Antibody ITGAE/3904R / CD103 Recombinant Antibody [clone ITGAE/3904R] (V8419)

ITGAE Antibody ITGAE/3904R Staining in Human Tonsil by IHC. Immunohistochemistry of FFPE human tonsil tissue using ITGAE Antibody (clone ITGAE/3904R) shows distinct brown staining in scattered lymphoid cells, with positive cells particularly evident within and around portions of the lymphoid compartment. The heterogeneous cellular distribution contrasts clearly with the densely hematoxylin-counterstained background and is consistent with CD103 expression in a subset of tonsillar lymphocytes. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 10mM Tris with 1mM EDTA for 20 min followed by cooling before testing. This pattern demonstrates CD103 detection using this CD103 Recombinant Antibody.

ITGAE Antibody ITGAE/3904R Purity and Integrity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free ITGAE Antibody (clone ITGAE/3904R) with 2 ug loaded per lane shows the expected recombinant immunoglobulin migration profiles under reducing (R) and non-reducing (NR) conditions. Under reducing conditions, prominent heavy and light chain bands are visible at approximately 50 kDa and 25 kDa, respectively, while the non-reduced antibody migrates predominantly as an intact species near 150 kDa. Limited additional staining is evident, supporting the integrity and purity of this CD103 Recombinant Antibody preparation.