CD103 Antibody ITGAE/2063 / Integrin Alpha E Monoclonal Antibody [clone ITGAE/2063] (V3848)

CD103 Antibody ITGAE/2063 Staining in Human Tonsil by IHC. Immunohistochemistry of FFPE human tonsil tissue using CD103 Antibody (clone ITGAE/2063) shows strong brown staining in scattered lymphoid cells distributed throughout the tissue, with numerous positive cells concentrated around portions of the lymphoid compartment. The staining is predominantly cellular and contrasts clearly with the densely packed hematoxylin-counterstained nuclei. Heat-induced epitope retrieval was performed by boiling sections in pH 6, 10 mM citrate buffer for 10-20 min followed by cooling at RT for 20 min. This pattern demonstrates detection of CD103-positive lymphocytes using this Integrin Alpha E Monoclonal Antibody.

CD103 Antibody ITGAE/2063 Staining in Human Colon by IHC. Immunohistochemistry of FFPE human colon tissue using CD103 Antibody (clone ITGAE/2063) shows prominent brown staining in numerous cells within the mucosal compartment, particularly among cells surrounding and between the colonic glands. The glandular epithelial cells are comparatively lightly stained, producing clear contrast with the CD103-positive cellular population. Heat-induced epitope retrieval was performed by boiling sections in pH 6, 10 mM citrate buffer for 10-20 min followed by cooling at RT for 20 min. The distribution is consistent with detection of mucosal lymphocytes using this Integrin Alpha E Monoclonal Antibody.

CD103 Antibody ITGAE/2063 Staining in Human Small Intestine by IHC. Immunohistochemistry of FFPE human small intestine tissue using CD103 Antibody (clone ITGAE/2063) shows distinct brown staining in scattered cells associated with the intestinal mucosa, including cells positioned around and between epithelial structures. The heterogeneous cellular distribution is consistent with the known localization of CD103-expressing mucosal lymphocytes. Heat-induced epitope retrieval was performed by boiling sections in pH 6, 10 mM citrate buffer for 10-20 min followed by cooling at RT for 20 min. This mucosal staining demonstrates detection of CD103-positive cells using this Integrin Alpha E Monoclonal Antibody.

CD103 Antibody ITGAE/2063 Protein Microarray Validated. HuProt protein microarray analysis containing more than 19,000 full-length human proteins demonstrates highly selective recognition of CD103 by CD103 Antibody (clone ITGAE/2063). CD103 ranks first with a Z-score of 95.59, compared with 7.61 for the next-ranked protein, CTBP1, producing an S-score of 87.99. The Z-score represents signal strength in standard deviations above the array mean, while the S-score measures separation from the next-ranked protein. The exceptionally large separation between CD103 and all other proteins provides strong evidence of target specificity for this Integrin Alpha E Monoclonal Antibody.

CD103 Antibody ITGAE/2063 Purity and Integrity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free CD103 Antibody (clone ITGAE/2063) shows a predominant intact immunoglobulin band at approximately 150 kDa under non-reducing conditions and the expected heavy and light chains at approximately 50 kDa and 25 kDa under reducing conditions. Two micrograms of antibody were loaded per lane. The limited additional staining and expected migration profiles support the integrity and purity of the preparation of this Integrin Alpha E Monoclonal Antibody.