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banner Search results for eEF1A

eEF1A Antibody / EEF1A1A/1B / Zebrafish

Catalog No : RZ1458
Applications : WB, IHC
Reactivity : Zebrafish
Format : Antigen affinity purified
Zebrafish eEF1A Antibody Skeletal Muscle IHC. Immunohistochemistry analysis of EEF1A1A/1B using anti-EEF1A antibody. EEF1A1A/1B was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-EEF1A1A/1B Antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using DAB as the chromogen.
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EEF1A1/2 Antibody

Catalog No : F54864
Applications : FACS, WB, IHC-P
Reactivity : Human
Format : Purified
Western blot testing of human 293T cell lysate with EEF1A1/2 antibody and a GADPH loading control Ab. Predicted molecular weight ~50 kDa.
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EEF1A1/2 Antibody

Catalog No : F54865
Applications : FACS, IF, IHC-P, WB
Reactivity : Human
Format : Purified
EEF1A1/2 Antibody Human MCF7 WB. Western blot analysis of EEF1A1/2 expression was performed using anti-EEF1A1/2 antibody in human MCF7 cell lysate. EEF1A1 and EEF1A2, also known as eukaryotic elongation factor 1 alpha proteins, are highly conserved GTP-binding factors that mediate aminoacyl-tRNA delivery during the elongation phase of protein synthesis. A prominent immunoreactive band is detected at approximately 50 kDa, corresponding to the predicted molecular weight of EEF1A1/2. Strong expression in MCF7 breast carcinoma cells is consistent with the abundant and ubiquitous nature of eEF1A proteins and their essential roles in translational control, cytoskeletal organization, and cellular proliferation. These results support the utility of EEF1A1/2 Antibody for studies of protein synthesis, cell growth, and cancer biology.
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EEF1A2 Antibody

Catalog No : R34872
Applications : WB, ELISA (peptide)
Reactivity : Human, Mouse, Rat
Pred. Reactivity :Cow, Dog
Format : Antigen affinity purified
Western blot testing of Jurkat lysate with EEF1A2 antibody at 0.5ug/ml. Predicted molecular weight: ~51kDa.
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EEF1A1 Antibody

Catalog No : FY13480
Applications : WB, ELISA, IF
Reactivity : Human, Mouse, Rat
Format : Lyophilized
EEF1A1 Antibody HeLa Cell IF. Immunofluorescent analysis of HeLa cells using EEF1A1 Antibody. Cells were subjected to enzyme-mediated antigen retrieval, blocked with goat serum, and incubated with EEF1A1 Antibody (5 ug/mL) overnight at 4°C. A Fluoro488-conjugated goat anti-rabbit secondary antibody was used to detect EEF1A1 (green), and nuclei were counterstained with DAPI (blue). Strong diffuse cytoplasmic fluorescence is observed with minimal nuclear staining, consistent with the expected localization of EEF1A1 as a cytosolic translation elongation factor. These results demonstrate that EEF1A1 Antibody, an Elongation Factor 1 Alpha 1 Antibody, specifically detects endogenous EEF1A1 in cultured human HeLa cells.
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