EEF1A1 Antibody / Elongation Factor 1 Alpha 1 Antibody (FY13480)

EEF1A1 Antibody HeLa Cell IF. Immunofluorescent analysis of HeLa cells using EEF1A1 Antibody. Cells were subjected to enzyme-mediated antigen retrieval, blocked with goat serum, and incubated with EEF1A1 Antibody (5 ug/mL) overnight at 4°C. A Fluoro488-conjugated goat anti-rabbit secondary antibody was used to detect EEF1A1 (green), and nuclei were counterstained with DAPI (blue). Strong diffuse cytoplasmic fluorescence is observed with minimal nuclear staining, consistent with the expected localization of EEF1A1 as a cytosolic translation elongation factor. These results demonstrate that EEF1A1 Antibody, an Elongation Factor 1 Alpha 1 Antibody, specifically detects endogenous EEF1A1 in cultured human HeLa cells.

EEF1A1 Antibody Human, Rat and Mouse Cell WB. Western blot analysis of EEF1A1 Antibody using lysates from 1) human HepG2 cells, 2) human HeLa cells, 3) rat NRK cells, and 4) mouse Neuro-2a cells. Samples (30 ug/lane) were separated on a 10% SDS-PAGE gel under reducing conditions, transferred to a nitrocellulose membrane, and probed with EEF1A1 Antibody (0.5 ug/mL), followed by an HRP-conjugated secondary antibody and ECL detection. A specific band is detected at approximately 50 kDa in all four cell lines, consistent with the predicted molecular weight of EEF1A1. These results demonstrate that EEF1A1 Antibody, an Elongation Factor 1 Alpha 1 Antibody, specifically detects endogenous EEF1A1 across human, rat, and mouse cell lines.