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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2571 to 2580
    NME2 Antibody / Nucleoside diphosphate kinase B (clone NME2/12953R)
    Catalog No : V5959
    Applications : WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western Blot Analysis of nm23-H2 / NME2 antibody (clone NME2/12953R). Human HeLa and human MCF7 whole cell lysates show a distinct immunoreactive band at approximately 17 kDa, consistent with the predicted molecular weight of NME2. The band appears as a single, sharp species in both cell lines with no significant non-specific background under reducing conditions, supporting specific detection of nm23-H2 protein.
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    PCNA Antibody / Proliferating cell nuclear antigen (clone PCNA/13725R)
    Catalog No : V5964
    Applications : WB
    Reactivity : Human, Mouse, Rat, Hamster, Rabbit, Guinea pig
    Format : Purified
    Recrabbitmono
    Western blot analysis of PCNA Proliferation Marker antibody in multi-species skin lysates. Western blot was performed using PCNA Proliferation Marker antibody (clone PCNA/13725R) on human skin, mouse skin, rat skin, hamster skin, guinea pig skin, and rabbit skin tissue lysates. A clear immunoreactive band is detected at approximately 29 kDa in all species tested, consistent with the predicted molecular weight of Proliferating Cell Nuclear Antigen. The conserved band size across species reflects the high sequence conservation of PCNA among mammals. The detected signal corresponds to the predicted molecular weight under reducing conditions. PCNA is a nuclear replication factor expressed in proliferating cells within skin tissue, and the consistent band pattern supports specific recognition of endogenous PCNA across multiple mammalian species.
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    Perforin Antibody / PRF1 (clone PRF1/9681R)
    Catalog No : V5975
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot analysis of Cytotoxic Lymphocyte Perforin Antibody in HDLM-2 cell lysate. HDLM-2 lysate shows a prominent band at approximately 70 kDa, consistent with the predicted molecular weight of Perforin-1. The signal appears as a strong, specific band under reducing SDS-PAGE conditions, with minor higher molecular weight signal that may represent protein aggregation or incompletely reduced species. The observed band aligns with endogenous PRF1 expression, which is expected in activated or cytotoxic lymphoid cell populations.
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    CD45 Antibody for IHC / Leukocyte Common Antigen (clone MSVA-045R)
    Catalog No : V5980
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    CD45 Antibody for IHC Tissue Microarray (TMA). Immunohistochemistry analysis of Protein tyrosine phosphatase receptor type C PTPRC, also known as Leukocyte common antigen CD45, in formalin-fixed paraffin-embedded human normal and cancer tissue microarrays using recombinant rabbit monoclonal CD45 antibody clone MSVA-045R. Tissue microarray (TMA) staining with HRP-DAB brown chromogen demonstrates robust membranous localization in lymphoid-rich tissues including tonsil, lymph node, spleen, and thymus, highlighting abundant leukocyte populations and normal immune architecture, while most non-hematopoietic epithelial and stromal tissues remain largely negative. Within tumor tissue microarrays, strong and diffuse staining is observed across multiple lymphoma subtypes, clearly identifying hematopoietic malignancies, whereas most non-hematologic tumors show minimal to no staining except for infiltrating immune cells within the tumor microenvironment. Evaluation across large TMA panels enables direct comparison of PTPRC expression across diverse tissue types under standardized conditions. The observed staining patterns align with reported PTPRC expression profiles in publicly available datasets including the Human Protein Atlas, supporting its use as a pan-leukocyte marker for immunohistochemistry studies.
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    PVALB Antibody / Parvalbumin (clone PVALB/12794R)
    Catalog No : V5983
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot analysis of Calcium-Binding Protein PV/PVALB antibody (clone PVALB/12794R) in brain tissue lysates from multiple species. Brain lysates from human, mouse, and rat show clear immunoreactive bands at approximately 12 kDa, consistent with the predicted molecular weight of Parvalbumin based on its amino acid sequence. No specific band is observed in hamster brain, guinea pig brain, or rabbit brain under the conditions tested. The detected bands migrate in the expected low molecular weight range for this small cytosolic EF-hand calcium-binding protein, supporting species-reactive detection of Parvalbumin in human, mouse, and rat brain tissue.
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    PVALB Antibody / Parvalbumin (clone PVALB/12610R)
    Catalog No : V5984
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of Fast-Spiking Interneuron Marker/Parvalbumin antibody (clone PVALB/12610R) in human kidney tissue. FFPE human kidney shows cytoplasmic HRP-DAB brown staining within renal tubular epithelial cells, consistent with Parvalbumin expression. Staining is localized predominantly to the cytoplasm of tubular structures, while adjacent glomerular elements and surrounding interstitial cells demonstrate minimal specific signal. The inset image represents a secondary antibody negative control in which PBS was used in place of the primary antibody and shows absence of specific staining. Heat induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes prior to immunostaining.
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    PMEL17 / Melanoma gp100 Antibody (clone PMEL/7554R)
    Catalog No : V5997
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Microvalidated
    Recrabbitmono
    Melanocyte Lineage Antibody / PMEL (clone PMEL/7554R). HuProt protein microarray specificity analysis using Melanocyte Lineage Antibody / PMEL clone PMEL/7554R against a protein array containing more than 21,000 full-length human proteins. The antibody shows strongest binding to PMEL (Premelanosome protein, also known as gp100), demonstrating selective recognition of the intended target compared with other proteins present on the array. Z score represents the signal strength of antibody binding to each protein on the array and is expressed as standard deviations above the mean signal across all proteins tested. The S score represents the difference between the Z score of the top-ranked target and the next highest signal and therefore indicates the relative specificity of the antibody for its intended protein target. An antibody is typically considered specific when the S score is greater than or equal to 2.5.
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    SOX9 Antibody for IHC / SRY-box transcription factor 9 (clone MSVA-709R)
    Catalog No : V5998
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    SOX9 IHC Antibody Tissue Microarray (TMA). Immunohistochemistry analysis of SRY-box transcription factor 9 SOX9 in formalin-fixed paraffin-embedded human normal and cancer tissue microarrays using recombinant rabbit monoclonal SOX9 antibody clone MSVA-709R. Tissue microarray (TMA) staining with HRP-DAB brown chromogen demonstrates distinct nuclear localization in epithelial progenitor compartments and glandular tissues, consistent with the role of SOX9 as a transcriptional regulator of differentiation and stem-like cell states. In normal tissue microarrays, nuclear staining is observed in gastrointestinal epithelium, pancreatic ducts, and reproductive tissues, while many fully differentiated tissues show reduced signal. Within tumor tissue microarrays, variable nuclear positivity is detected across multiple carcinomas, with stronger staining often associated with regions of glandular differentiation and cellular plasticity. Evaluation across large TMA panels enables direct comparison of SOX9 expression across diverse tissue types under standardized conditions. The observed staining patterns align with reported SOX9 expression profiles in the Human Protein Atlas.
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    STAT6 Antibody / Signal transducer and activator of transcription 6 (clone STAT6/12798R)
    Catalog No : V6003
    Applications : IHC, Knockdown, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Signal Transducer 6 Antibody Knockdown Validation WB. Western blot analysis of STAT6 expression in wild-type (WT) and STAT6 shRNA knockdown HeLa cells using Signal Transducer 6 antibody clone STAT6/12798R. Lane 1: WT lysate, Lane 2: knockdown lysate. The band at approximately 90-100 kDa is reduced in knockdown cells, supporting target-specific detection. Hsp90 alpha is shown as a loading control.
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    BST2 Antibody / Bone marrow stromal antigen 2 (clone BST2/13681R)
    Catalog No : V6005
    Applications : ELISA, IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of BST2/Bone marrow stromal antigen 2 antibody in human ovary tissue (clone BST2/13681R). FFPE human ovary sections demonstrate HRP-DAB brown membranous and cytoplasmic staining in epithelial cells lining ovarian structures, with strong cell surface localization consistent with Bone marrow stromal antigen 2 expression. Surrounding stromal areas show comparatively weaker staining. The inset shows PBS used in place of primary antibody as a secondary antibody negative control, demonstrating absence of specific brown staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.
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