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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2491 to 2500
    Bcl-X Antibody / BCL2L1 (clone 31B78)
    Catalog No : FY13325
    Applications : WB, IHC, IP
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    All lanes use the BCL2L1 antibody at 1:1K dilution for 1 hour at room temperature.
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    UBF1 Antibody / Upstream binding factor 1 / UBTF (clone 32U20)
    Catalog No : FY13331
    Applications : WB, IHC
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the UBTF antibody at 1:1000 dilution for 1 hour at room temperature. A predominant band is detected at an approximately 89 kDa in all samples, consistent with full length UBTF. Lysates also show a weaker band near 30 kDa, which likely represents a proteolytic fragment of UBTF that retains the antibody epitope rather than a distinct isoform.
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    ROCK1 Antibody / Rho associated coiled coil containing protein kinase 1 (clone 32R84)
    Catalog No : FY13332
    Applications : WB, IHC, IP, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the ROCK1 antibody at 1:2000 dilution for 1 hour at room temperature. A predominant band is detected at an approximately 158 kDa in all samples, consistent with full length ROCK1. An additional weaker band is observed just above the 55 kDa marker, which likely represents a proteolytic fragment of ROCK1 generated during cell stress or sample preparation rather than a distinct isoform.
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    HSC70 Antibody / Heat shock protein 70 / HSPA8 (clone 31H87)
    Catalog No : FY13333
    Applications : WB, IHC, ICC, IF, IP, FACS
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Hsp70 using anti-Hsp70 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp70 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The predicted molecular weight of Hsp70 is ~70 kDa and ~64 kDa (two isoforms).
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    TCEA1 Antibody / Transcription elongation factor A protein 1 (clone 29T88)
    Catalog No : FY13340
    Applications : WB, IHC, ICC, IF, IP
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Immunohistochemical staining of paraffin-embedded mouse large intestine tissue with TCEA1 antibody.
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    TMEM43 Antibody / Transmembrane protein 43 / LUMA (clone 30T30)
    Catalog No : FY13343
    Applications : WB, IHC
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of TMEM43 using anti-TMEM43 antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM43 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band is detected at an approximately 40 kDa in all samples, slightly below the predicted ~45 kDa size but consistent with the apparent molecular weight typically observed for the integral membrane protein TMEM43 under denaturing conditions.
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    HMGCL Antibody / Hydroxymethylglutaryl CoA lyase (clone 30H50)
    Catalog No : FY13344
    Applications : WB, IHC
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of HMGCL expression in human A431 cell lysate using HMGCL antibody. Predicted molecular weight ~34 kDa.
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    Phospho-GLUT4 (pSer488) Antibody / SLC2A4 (clone 32S34)
    Catalog No : FY13345
    Applications : WB
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    All lanes use the Phospho-GLUT4 (pSer488) antibody at 1:1000 dilution for 1 hour at room temperature. Predicted molecular weight ~55 kDa and ~44 kDa (two isoforms).
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    Phospho-RIP3 (pSer232) Antibody / RIPK3 (clone 31R36)
    Catalog No : FY13346
    Applications : WB
    Reactivity : Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Phospho-RIP3 (S232) using anti-Phospho-RIP3 (pS232) antibody. Lane 1: rat spleen tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse L929 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-RIP3 (S232) antibody (S232) at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected molecular weight of Phospho-RIP3 (S232) is at 50 kDa.
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    Phospho-Tau (pSer202) Antibody / MAPT (clone 31M22)
    Catalog No : FY13349
    Applications : WB
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Phospho-Tau (pS202) expression in mouse hippocampus cell lysate using Phospho-Tau (pSer202) antibody. The expected molecular weight of phosphorylated Tau (Ser202) is approximately 45-70 kDa, corresponding to the major Tau isoforms.
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