KRT20 Antibody for IF / Cytokeratin 20 Immunofluorescence Antibody [clone CDI-11] (FY13371)

KRT20 Antibody for IHC. Immunohistochemistry analysis of paraffin-embedded human colon tissue using KRT20 antibody. HRP-DAB brown chromogenic staining highlights cytoplasmic Keratin 20 / Cytokeratin 20 (KRT20) expression in colonic epithelial cells lining the glandular structures, while surrounding stromal cells remain largely negative. The staining pattern reflects the expected epithelial localization of this intermediate filament protein in gastrointestinal mucosa.

Western blot testing of mouse and rat samples using the KRT20 antibody at 1:5000 dilution for 1 hour at room temperature. The expected molecular weight of KRT20 is at 46 kDa.

Immunofluorescent analysis of human Hela cells using KRT20 antibody (green) and DAPI nuclear stain (blue).

KRT20 Antibody for IF. Immunofluorescent analysis of human HeLa cells using KRT20 Antibody for IF (green) at 1:50 dilution. Cytoplasmic fluorescence highlights Keratin 20 / Cytokeratin 20 (KRT20) intermediate filament staining within epithelial cells. Phalloidin-TRITC (red) labels actin cytoskeletal filaments and DAPI (blue) marks cell nuclei. The merged image demonstrates cytoplasmic localization of KRT20 consistent with epithelial intermediate filament organization observed by fluorescence microscopy.

Western blot analysis of Cytokeratin 20 using anti-KRT20 antibody. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT20 antibody at 1:1000 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected molecular weight of KRT20 is at 46 kDa.

Immunohistochemical staining of Cytokeratin 20 using anti-KRT20 antibody. KRT20 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KRT20 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

Immunohistochemical staining of Cytokeratin 20 using anti-KRT20 antibody. KRT20 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KRT20 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.