KRT20 Antibody / Cytokeratin 20 / CK20 [clone CDI-11] (FY13371)

Immunohistochemical staining of paraffin-embedded human colon tissue using KRT20 antibody.

Western blot testing of mouse and rat samples using the KRT20 antibody at 1:5000 dilution for 1 hour at room temperature. The expected molecular weight of KRT20 is at 46 kDa.

Immunofluorescent analysis of human Hela cells using KRT20 antibody (green) and DAPI nuclear stain (blue).

Immunofluorescent analysis using the KRT20 antibody (green) at 1:50 dilution.

Western blot analysis of Cytokeratin 20 using anti-KRT20 antibody. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT20 antibody at 1:1000 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected molecular weight of KRT20 is at 46 kDa.

Immunohistochemical staining of Cytokeratin 20 using anti-KRT20 antibody. KRT20 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KRT20 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

Immunohistochemical staining of Cytokeratin 20 using anti-KRT20 antibody. KRT20 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KRT20 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.