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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2391 to 2400
    NUP107 Antibody / Nucleoporin 107 (clone 29N68)
    Catalog No : FY12917
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Nup107 expression in human 293 cell lysate using NUP107 antibody. Expected molecular weight ~107 kDa.
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    Histone H3 (di methyl K4) Antibody / HIST1H3A (clone 31H99)
    Catalog No : FY12922
    Applications : WB, IHC, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    All lanes use the Histone H3 (di methyl K4) antibody at 1:1000 dilution for 1 hour at room temperature.
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    MAPKAPK3 Antibody / Mitogen activated protein kinase activated protein kinase 3 (clone 30M15)
    Catalog No : FY12924
    Applications : WB, IP
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Immunoprecipitation analysis using the MAPKAPK3 antibody at 1:50 dilution. Western blot at 1:500 dilution. Predicted molecular weight ~43 kDa.
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    IL9 Antibody / Interleukin 9 (clone 31I10)
    Catalog No : FY12940
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of recombinant Interleukin 9 using IL9 antibody.
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    Phospho-LRP6 (pSer1490) Antibody / Low density lipoprotein receptor related protein 6 (clone 31L42)
    Catalog No : FY12941
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Phospho-LRP6 (pS1490) expression in lysate from human HeLa cells treated with Calyculin, using Phospho-LRP6 (pSer1490) antibody. A strong band is detected at ~220-230 kDa, consistent with the mature, heavily N-glycosylated and hyperphosphorylated form of LRP6, which migrates slower than the ~180 kDa unglycosylated core polypeptide.
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    Phospho-Tuberin (pSer939) Antibody / TSC2 (clone 32T70)
    Catalog No : FY12943
    Applications : WB, IHC
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Immunohistochemical staining of Tuberin/TSC2 (Phospho-S939) using anti-Phospho-Tuberin (pSer939) antibody. Tuberin/TSC2 (Phospho-S939) was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-Phospho-Tuberin (Ser939) antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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    Phospho-CrkL (pTyr207) Antibody / Crk like protein (clone 32C08)
    Catalog No : FY12948
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    All lanes use the Phospho-CrkL (pTyr207) antibody at 1:1000 dilution for 1 hour at room temperature. A tight doublet is detected at ~40 kDa in K562 cells, running above the ~34 kDa predicted mass. The upward shift and doublet pattern are consistent with mono- and hyper-phosphorylated CrkL species, which migrate slower on SDS-PAGE than total CrkL.
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    SYNE3 Antibody / Nesprin 3 (clone 30S37)
    Catalog No : FY12952
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Nesprin 3 expression in human 293 cell lysate using SYNE3 antibody. A single band is detected at ~100 kDa, running slightly below the ~112 kDa prediction. The migration is consistent with the reported nesprin-3 isoform pattern (long form ~108-112 kDa, short form ~95-105 kDa) and the faster apparent mobility of coiled-coil nesprins on SDS-PAGE.
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    JPT1 Antibody / HN1 / Hematological and neurological expressed 1 (clone 31J27)
    Catalog No : FY12957
    Applications : FACS, IF, ICC, WB
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the HN1 antibody at 1:1000 dilution for 1 hour at room temperature. Predicted molecular weight: 16-20 kDa (two isoforms).
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    PSIP1 Antibody / PC4 and SFRS1-interacting protein / LEDGF (clone 31P66)
    Catalog No : FY12959
    Applications : WB, IHC, ICC, IF
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the PSIP1 antibody at 1:1000 dilution for 1 hour at room temperature. A doublet is observed at ~50-55 kDa corresponding to the unmodified p75 and p52 isoforms, with an additional band at ~80–90 kDa representing post-translationally modified or complexed PSIP1 forms described in the literature.
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