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Phospho Antibody / Antibodies

Home >> Antibodies >> Phospho Antibody / Antibodies

Phospho Antibody / Antibodies

Phospho antibodies available from NSJ Bioreagents:
Found all antibodies, displaying 321 to 328
Phospho-CaMKII alpha (pT286) Antibody / CAMK2A (clone 31C26)
Catalog No : FY12828
Applications : WB, IHC
Reactivity : Mouse, Rat
Format : Liquid
Recrabbitmono
Phospho-CaMKII alpha (pThr286) western blot of mouse brain using Phospho-CaMKII alpha (pT286) antibody. Lane 1, strong ~50/55 kDa doublet consistent with different phosphorylation states of CaMKII alpha; lane 2, lambda phosphatase treatment abolishes the signal, confirming phospho-specificity.
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Phospho-AS160 (pThr642) Antibody / TBC1D4 (clone 31T47)
Catalog No : FY12844
Applications : WB
Reactivity : Human
Format : Liquid
Recrabbitmono
Western blot analysis of Phospho-AS160 (pT642) expression in lysate from human 293T cells treated with insulin, using Phospho-AS160 (pThr642) antibody. Predicted molecular weight ~147 kDa, commonly observed at ~160 kDa.
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Phospho-PRAS40 (pThr246) Antibody / AKT1S1 (clone 32A68)
Catalog No : FY12850
Applications : WB
Reactivity : Human
Format : Liquid
Recrabbitmono
Mouse NIH 3T3 cell lysate tested with the Phospho-PRAS40 (pThr246) antibody at 1:1000 dilution for 1 hour at room temperature. Predicted molecular weight ~40 kDa.
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Phospho-LRP6 (pSer1490) Antibody / Low density lipoprotein receptor related protein 6 (clone 31L42)
Catalog No : FY12941
Applications : WB
Reactivity : Human
Format : Liquid
Recrabbitmono
Western blot analysis of Phospho-LRP6 (pS1490) expression in lysate from human HeLa cells treated with Calyculin, using Phospho-LRP6 (pSer1490) antibody. A strong band is detected at ~220-230 kDa, consistent with the mature, heavily N-glycosylated and hyperphosphorylated form of LRP6, which migrates slower than the ~180 kDa unglycosylated core polypeptide.
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Phospho-Tuberin (pSer939) Antibody / TSC2 (clone 32T70)
Catalog No : FY12943
Applications : WB, IHC
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono
Immunohistochemical staining of Tuberin/TSC2 (Phospho-S939) using anti-Phospho-Tuberin (pSer939) antibody. Tuberin/TSC2 (Phospho-S939) was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-Phospho-Tuberin (Ser939) antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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Phospho-CrkL (pTyr207) Antibody / Crk like protein (clone 32C08)
Catalog No : FY12948
Applications : WB
Reactivity : Human
Format : Liquid
Recrabbitmono
All lanes use the Phospho-CrkL (pTyr207) antibody at 1:1000 dilution for 1 hour at room temperature. A tight doublet is detected at ~40 kDa in K562 cells, running above the ~34 kDa predicted mass. The upward shift and doublet pattern are consistent with mono- and hyper-phosphorylated CrkL species, which migrate slower on SDS-PAGE than total CrkL.
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Phospho-p38 (pThr180) Antibody / MAPK14 (clone 31M76)
Catalog No : FY12991
Applications : WB, IHC
Reactivity : Human
Format : Liquid
Recrabbitmono
Western blot using the Phospho-p38 (pThr180) antibody and MCF-7 cell lysate shows a predominant band at ~38-40 kDa consistent with p38 alpha, with a faint higher species (~41-42 kDa) attributable to p38 beta or a more highly phosphorylated pool.
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Phospho-SIRT1 (pThr530) Antibody (clone HAI-19)
Catalog No : FY13407
Applications : WB
Reactivity : Human
Format : Liquid
Recrabbitmono
Western blot analysis of Phospho-SIRT1 (pThr530) expression in (1) 293T cell lysate and (2) lysate from 293T cells treated with Calyculin A, using Phospho-SIRT1 (pThr530) antibody.
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