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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
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Signal Transduction Antibodies

Home >> Antibodies >> Signal Transduction Antibodies

Signal Transduction Antibodies

NSJ Bioreagents offers the following antibodies in the area of Signal Transduction:
Found all antibodies, displaying 10951 to 10959
EGLN1 Antibody / Egl nine homolog 1 / PHD2
Catalog No : FY13439
Applications : WB, FACS, IP
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunoprecipitation and western blot analysis of Egl nine homolog 1 using EGLN1 antibody. Lane 1: input human A549 whole cell lysates (30 ug); Lane 2: immunoprecipitation performed using rabbit control IgG with A549 whole cell lysates; Lane 3: immunoprecipitation performed using EGLN1 antibody with A549 whole cell lysates (500 ug input, 2 ug antibody). Following immunoprecipitation, proteins were resolved by SDS-PAGE and transferred to a membrane. A distinct band is detected at approximately 46 kDa in the EGLN1 antibody immunoprecipitation lane, consistent with the predicted molecular weight of Egl nine homolog 1. The control IgG lane shows no corresponding signal, supporting specific enrichment of EGLN1 from A549 cell lysates.
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RXFP1 Antibody / Relaxin receptor 1
Catalog No : FY13440
Applications : WB, ELISA
Reactivity : Human
Format : Lyophilized
Western blot analysis of Relaxin receptor 1 using RXFP1 antibody. Lane 1: human THP-1 whole cell lysates; Lane 2: human HEL whole cell lysates. A prominent band is detected at approximately 85 kDa, consistent with the predicted molecular weight of Relaxin receptor 1. Additional lower-molecular-weight bands are observed at approximately 65-70 kDa and ~35 kDa, which may reflect partially glycosylated receptor forms or proteolytic fragments commonly reported for GPCR family members such as RXFP1. The overall banding pattern is consistent with detection of RXFP1 in human cell lysates.
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PDE4A Antibody / cAMP-specific 3,5-cyclic phosphodiesterase 4A
Catalog No : FY13442
Applications : IF, IHC
Reactivity : Human
Format : Lyophilized
Immunohistochemistry analysis of Phosphodiesterase 4A using PDE4A antibody. Phosphodiesterase 4A expression was examined in a paraffin-embedded section of human colon cancer tissue. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with PDE4A antibody overnight at 4°C. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen, followed by hematoxylin counterstaining.
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DUSP2 Antibody / Dual specificity protein phosphatase 2
Catalog No : FY13443
Applications : IF, IHC, WB, FACS
Reactivity : Human
Format : Lyophilized
Immunofluorescence localization of DUSP2 in HeLa cells. DUSP2 expression was analyzed in cultured HeLa cells by immunofluorescence. Cells were fixed, subjected to enzymatic antigen retrieval, and incubated with an anti-DUSP2 antibody together with an alpha-tubulin antibody as a cytoskeletal marker. Fluorescent secondary antibodies were used for detection. DUSP2 staining is visualized in green, alpha-tubulin in red.
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TBX5 Antibody / T-box protein 5
Catalog No : FY13444
Applications : IF, IHC, WB, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescence analysis of TBX5 using anti-TBX5 antibody. TBX5 expression was examined in a paraffin-embedded section of human lung cancer tissue. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0), followed by blocking with goat serum. The tissue section was incubated with a rabbit anti-TBX5 antibody overnight at 4°C, then stained with a Cy3-conjugated goat anti-rabbit secondary antibody. Nuclei were counterstained with DAPI. Fluorescent signals were visualized by fluorescence microscopy, revealing prominent nuclear localization of TBX5 consistent with its role as a transcription factor.
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FFAR2 Antibody / Free fatty acid receptor 2
Catalog No : FY13446
Applications : WB, FACS, ELISA
Reactivity : Human
Format : Lyophilized
Flow cytometry analysis of fixed human ThP-1 cells with FFAR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FFAR2 antibody.
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PDGFD Antibody / Platelet-derived growth factor D
Catalog No : FY13449
Applications : WB, IHC, IP, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescence analysis of Platelet-derived growth factor D using PDGF-D antibody. Platelet-derived growth factor D expression was examined in a paraffin-embedded section of mouse kidney tissue. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with PDGF-D antibody (red) overnight at 4C. Immunoreactivity was visualized using fluorescence detection, and nuclei were counterstained with DAPI (blue).
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PELO Antibody / Protein pelota homolog
Catalog No : FY13452
Applications : WB, IHC
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunohistochemistry analysis of Protein pelota homolog using PELO antibody. PELO expression was examined in a paraffin-embedded section of rat kidney tissue. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with PELO antibody overnight at 4C. Immunoreactivity is observed predominantly in renal tubular epithelial cells, with minimal staining in surrounding stromal areas. Detection was visualized using an HRP-based detection system with DAB chromogen, followed by hematoxylin counterstaining.
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PDE8B Antibody / Phosphodiesterase 8B
Catalog No : FY13453
Applications : WB, IF
Reactivity : Human
Format : Lyophilized
Immunofluorescence analysis of Phosphodiesterase 8B using PDE8B antibody. PDE8B expression was examined in cultured HeLa cells. Enzymatic antigen retrieval was performed prior to staining. Cells were blocked with normal goat serum and incubated with PDE8B antibody (green) overnight at 4C. Immunoreactivity was visualized by fluorescence microscopy, and nuclei were counterstained with DAPI (blue).
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