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Home >> Antibodies >> TFF1 Antibody / Trefoil factor 1

TFF1 Antibody / Trefoil factor 1 [clone MSVA-482M] (V6118)

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  Catalog No Formulation Size Price (USD)  
Image V6118-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6118-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Trefoil factor 1 Mouse Monoclonal Antibody (MSVA-482M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name MSVA-482M
UniProt P04155
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This TFF1/Trefoil factor 1 antibody is available for research use only.
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Application Notes

1. Optimal dilution of the TFF1/Trefoil factor 1 antibody should be determined by the researcher.

2. This TFF1/Trefoil factor 1 antibody is recombinantly produced by expression in CHO cells.

Immunogen

A recombinant fragment (around amino acids 1-84) of human TFF1 protein (exact sequence is proprietary) was used as the immuongen for the TFF1/Trefoil factor 1 antibody.

Storage

TFF1/Trefoil factor 1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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