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Home >> Antibodies >> Desmin Antibody

Desmin Antibody [clone D33] (V3200)

  Catalog No Formulation Size Price (USD)  
Image V3200-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3200-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3200SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Bulk quote request
IHC testing of FFPE human leiomyosarcoma with Desmin antibody (clone D33). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name D33
Purity Protein A/G affinity
UniProt P17661
Localization Cytoplasmic, membranous
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Desmin antibody is available for research use only.
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Description

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain α-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Application Notes

Optimal dilution of the Desmin antibody should be determined by the researcher.

Immunogen

Human leiomyoma proteins were used as the immunogen for the Desmin antibody.

Storage

Store the Desmin antibody at 2-8oC.

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