Desmin Antibody for IF / DES Immunofluorescence Antibody (R31530)

Desmin Antibody for IF Mouse Heart Immunofluorescence. Immunofluorescence analysis of Desmin (DES) expression in FFPE mouse heart tissue using Desmin Antibody for IF demonstrates strong cytoplasmic filamentous red fluorescence in cardiomyocytes, consistent with Desmin localization within the muscle cytoskeletal network, while nuclei are counterstained with DAPI (blue). The staining pattern highlights organized myofibrillar structure and muscle cell architecture, with signal confined to cardiac muscle cells and minimal background in non-muscle components. Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes prior to antibody incubation.

Desmin Antibody for IF Mouse Skeletal Muscle Immunofluorescence. Immunofluorescence analysis of Desmin (DES) expression in FFPE mouse skeletal muscle tissue using Desmin Antibody for IF demonstrates strong cytoplasmic filamentous red fluorescence outlining muscle fibers, consistent with Desmin localization within the myofibrillar cytoskeletal network, while nuclei are counterstained with DAPI (blue). The staining pattern highlights muscle fiber architecture and clear delineation of individual myocytes with minimal background signal. Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes prior to antibody incubation.

Desmin Antibody for IF Rat Heart Immunofluorescence. Immunofluorescence analysis of Desmin (DES) expression in FFPE rat heart tissue using Desmin Antibody for IF demonstrates strong cytoplasmic filamentous red fluorescence in cardiomyocytes, consistent with Desmin localization within the cardiac muscle cytoskeletal network, while nuclei are counterstained with DAPI (blue). The staining pattern highlights organized myofibrillar structure and muscle fiber alignment with minimal background in non-muscle components. Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes prior to antibody incubation.

Desmin Antibody for IF Rat Skeletal Muscle Immunofluorescence. Immunofluorescence analysis of Desmin (DES) expression in FFPE rat skeletal muscle tissue using Desmin Antibody for IF demonstrates strong cytoplasmic filamentous red fluorescence outlining muscle fibers, consistent with Desmin localization within the myofibrillar cytoskeletal network, while nuclei are counterstained with DAPI (blue). The staining pattern highlights muscle fiber organization and clear delineation of individual myocytes with minimal background signal. Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes prior to antibody incubation.

IHC staining of FFPE human lung cancer tissue with Desmin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat skeletal muscle tissue with Desmin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of frozen rat heart tissue with Desmin antibody.

Western blot testing of 1) rat heart, 2) rat skeletal muscle, 3) rat C2C12, 4) mouse heart and 5) mouse skeletal muscle tissue lysate with Desmin antibody. Predicted molecular weight ~54 kDa.