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Home >> Antibodies >> Zebrafish Desmin Antibody / DES Antibody

Zebrafish Desmin Antibody / DES Antibody (RZ1441)

  Catalog No Formulation Size Price (USD)  
Image RZ1441 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Zebrafish Desmin Antibody by IHC in zebrafish muscle. Immunohistochemistry of FFPE zebrafish muscle tissue using Zebrafish Desmin Antibody at 2 ug/ml. Brown DAB staining is observed along elongated muscle fibers, consistent with the cytoplasmic distribution of the DESMA intermediate filament protein. Sections underwent heat-induced antigen retrieval in EDTA buffer, pH 8.0, followed by overnight primary antibody incubation at 4oC. This DES Antibody detects a structural protein involved in muscle fiber organization and cytoskeletal stability.
Zebrafish Desmin Antibody by IF in zebrafish muscle. Immunofluorescence staining of FFPE zebrafish muscle tissue using Zebrafish Desmin Antibody at 5 ug/ml. Green fluorescence highlights elongated muscle fibers and filament-associated structures, while DAPI counterstaining identifies nuclei in blue. Heat-induced antigen retrieval was performed in EDTA buffer, pH 8.0, followed by overnight primary antibody incubation at 4oC. Fluoro488-conjugated goat anti-rabbit IgG was used at 1:500. This DES Antibody recognizes a muscle-associated intermediate filament protein involved in maintaining cellular architecture.
Zebrafish Desmin Antibody by WB in zebrafish head and embryo lysates. Western blot analysis of zebrafish head (lane 1) and embryo (lane 2) tissue lysates using Zebrafish Desmin Antibody at 0.5 ug/ml. Prominent immunoreactive bands are detected at approximately 45-50 kDa in both samples, reasonably consistent with the predicted DESMA molecular weight of 54 kDa. The difference between observed and predicted molecular weights may reflect protein processing or electrophoretic migration. This DES Antibody detects a type III intermediate filament protein involved in muscle development and cytoskeletal organization.
Zebrafish Desmin Antibody by WB in zebrafish head, whole female, whole male, and embryo lysates. Western blot analysis using Zebrafish Desmin Antibody at 0.5 ug/ml. Lane 1: zebrafish head; lane 2: whole female zebrafish; lane 3: whole male zebrafish; lane 4: zebrafish embryo. Each lane contained 30 ug protein separated on a 10% SDS-PAGE gel under reducing conditions. Immunoreactive bands are observed at approximately 45-50 kDa, compared with the predicted DESMA molecular weight of 54 kDa. This DES Antibody recognizes a muscle-specific intermediate filament protein involved in cytoskeletal organization.
Species Reactivity Zebrafish
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Buffer Lyophilized from a buffered saline solution containing 2% trehalose. Reconstitute with 0.2 mL distilled water to yield a final antibody concentration of 500 ug/mL.
UniProt F1R8W4
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Limitations This Zebrafish Desmin Antibody / DES Antibody is available for research use only.
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Description

Zebrafish Desmin Antibody / DES Antibody is designed for the detection and study of DESMA, the zebrafish ortholog of human Desmin (DES), a type III intermediate filament protein that serves as a major structural component of muscle cells. Zebrafish Desmin Antibody enables investigation of cytoskeletal networks that maintain cellular architecture and mechanical stability within skeletal, cardiac, and smooth muscle tissues. Through these functions, Desmin contributes to muscle organization, tissue integrity, and normal vertebrate development.

Desmin belongs to the intermediate filament family of cytoskeletal proteins and forms supportive intracellular networks that help preserve cell shape and structural cohesion. These filament systems connect contractile elements, anchor organelles, and provide mechanical resilience within muscle cells. Zebrafish Desmin Antibody is useful for studying the molecular architecture of muscle tissue and the mechanisms that coordinate muscle assembly and maintenance throughout development and adulthood.

Zebrafish Desmin Antibody supports research into muscle development and vertebrate physiology using one of the most widely utilized animal models in developmental biology. Desmin expression is highly conserved among vertebrates and plays important roles during myogenesis, muscle maturation, and organization of contractile tissues. Studies utilizing Zebrafish Desmin Antibody can help characterize expression patterns associated with muscle formation, growth, and structural specialization.

Beyond developmental biology, Desmin has attracted significant interest in cardiovascular biology, skeletal muscle physiology, tissue remodeling, and cytoskeletal organization. Because intermediate filaments provide essential structural support within muscle cells and contribute to intracellular connectivity, Desmin remains an important target for investigations examining how cytoskeletal networks maintain tissue function and respond to physiologic stress. Zebrafish Desmin Antibody supports these studies by enabling characterization of Desmin expression in developmental and adult tissues.

Zebrafish Desmin Antibody is useful for investigating muscle biology, intermediate filament organization, cytoskeletal regulation, tissue development, and vertebrate physiology. Researchers utilize Zebrafish Desmin Antibody to better understand molecular mechanisms governing muscle structure, cellular architecture, tissue integrity, and cytoskeleton-dependent regulatory pathways.

This antibody can be compared with our Desmin Antibody (clone DES/2960R) for detection of desmin as a muscle marker with validation supported by gene knockdown.

This Zebrafish antibody is part of a broader Zebrafish / Danio rerio antibody panel offered by NSJ Bioreagents.

Application Notes

The optimal working dilution of the Zebrafish Desmin Antibody / DES Antibody should be determined empirically by the investigator.

Immunogen

An E.coli-derived Zebrafish DESMA recombinant protein (amino acids S71-K370) was used as the immunogen for the Zebrafish DESMA / Desmin Antibody.

Storage

After reconstitution, the Zebrafish DESMA / Desmin Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Zebrafish Desmin Antibody, Zebrafish DES Antibody, Zebrafish DESMA Antibody, Zebrafish Intermediate Filament Protein Antibody, Zebrafish Muscle Cytoskeleton Protein Antibody, Zebrafish Muscle Structural Protein Antibody, Zebrafish Myocyte Intermediate Filament Antibody

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