- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Human Nuclear Antigen Antibody [clone 235-1] (V2345)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V2345-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 539 | |
|
|
V2345-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 249 | |
|
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V2345SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 |
| Species Reactivity | Human. Does not react with mouse, rat. |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | 235-1 |
| Purity | Protein G affinity chromatography |
| Buffer | 1X PBS, pH 7.4 |
| Localization | Nuclear |
| Applications | Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml |
| Limitations | This Nuclear Antigen antibody is available for research use only. |
|
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Human Nuclear Antigen antibody clone 235-1 is a monoclonal antibody that detects a broadly expressed nuclear antigen present in nearly all human cells. This makes it an ideal marker for labeling nuclei in a wide range of tissues and cell lines. Unlike antibodies targeting lineage-specific proteins, Human Nuclear Antigen antibody clone 235-1 is valued for its universal reactivity, offering consistent nuclear labeling that can be used as a counterstain, reference marker, or cell identification tool. NSJ Bioreagents provides this antibody for reliable and reproducible nuclear staining in developmental biology, oncology, and pathology research.
Human Nuclear Antigen antibody clone 235-1 produces strong nuclear staining across epithelial, mesenchymal, and hematopoietic cells, delivering consistent results regardless of tissue type. In diagnostic and research applications, this antibody is commonly employed to highlight nuclei when assessing tissue architecture, tumor cell density, or overall cell number. Its broad reactivity makes it useful in multiplex panels where nuclear labeling is required alongside other lineage-specific antibodies.
In cancer biology, Human Nuclear Antigen antibody clone 235-1 has been widely applied to identify tumor cells and distinguish them from surrounding stroma or infiltrating immune cells. By providing a reliable nuclear counterstain, this antibody assists in quantifying proliferative indices and tumor burden in tissue samples. Researchers also use it to assess nuclear morphology in malignancies, helping to evaluate differentiation and atypia.
Beyond oncology, this antibody is applied in developmental biology to track nuclear distribution during embryogenesis and organogenesis. Its universal nuclear labeling enables clear visualization of cell positioning and tissue organization at multiple stages of development.
In regenerative medicine and stem cell research, Human Nuclear Antigen antibody clone 235-1 is valuable for confirming cell identity and monitoring differentiation. Because the antibody marks nuclei in virtually all human cells, it provides a dependable reference point in complex experiments where multiple markers are combined.
Technically, clone 235-1 has been validated in both tissue-based and cell-based systems, producing sharp nuclear signals with minimal background. Its robust performance has made it a standard nuclear marker in immunohistochemistry and related studies. Alternate names include nuclear marker antibody, human nuclear protein antibody, and nuclear localization antibody.
Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nuclear Antigen antibody to be titered up or down for optimal performance.
Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear Antigen antibody.
Store the Nuclear Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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