Human IgG Antibody IG266 / Mouse Monoclonal [clone IG266] (V2140)

Human IgG Antibody IG266 Tonsil IHC. Immunohistochemistry testing of human tonsil stained with Human IgG antibody (IG266). HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool before testing.

Human IgG Antibody IG266 Tonsil Tissue IHC. Immunohistochemistry staining of FFPE human tonsil with Human IgG antibody clone IG266. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IgG antibody (clone IG266). These results demonstrate the foremost specificity of the IG266 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free anti-IgG antibody (clone IG266) as confirmation of integrity and purity.

Human IgG Antibody IG266 Serum WB. Western blot analysis was performed using IgG Antibody clone IG266 on human serum lysate. A distinct immunoreactive band is detected at approximately 50-55 kDa, consistent with the expected molecular weight of the human IgG heavy chain. The blot exhibits a strong, specific signal with minimal background, supporting reliable detection of endogenous human IgG in serum samples. These results demonstrate that this IGHG1 Antibody is suitable for Western blot detection of human IgG in serum.