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Home >> Antibodies >> Anti-IgG Antibody

Anti-IgG Antibody [clone ICO-97] (V2141)

  Catalog No Formulation Size Price (USD)  
Image V2141-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2141-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2141SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name ICO-97
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 3500
Localization Cytoplasm, Cell Surface and Secreted
Applications ELISA (order BSA/sodium azide-free format for coating) :
Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Limitations This anti-IgG antibody is available for research use only.
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Description

This antibody recognizes a protein of 75kDa, identified as the gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-HodgkinÂ’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-IgG antibody to be titered up or down for optimal performance.

Immunogen

Purified human IgG heavy chain was used as the immunogen for this anti-IgG antibody.

Storage

Store the anti-IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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