- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD45RA Antibody / Protein Tyrosine Phosphatase Receptor Type C Isoform RA [clone 158-4D3] (V2238)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V2238-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V2238-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V2238SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 | |
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V2238IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 559 |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2a, kappa |
| Clone Name | 158-4D3 |
| Purity | Protein G affinity chromatography |
| Gene ID | 5788 |
| Localization | Cell surface |
| Applications | Flow Cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This CD45RA antibody is available for research use only. |
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CD45RA antibody targets Protein Tyrosine Phosphatase Receptor Type C isoform RA, a transmembrane protein tyrosine phosphatase encoded by the PTPRC gene and expressed on the surface of hematopoietic cells. CD45RA represents a specific splice isoform of the CD45 family, generated through alternative exon usage, and is predominantly localized to the plasma membrane of leukocytes. This isoform is most well known for its expression on naive T lymphocytes, where it serves as a defining immunophenotypic marker used to distinguish naive from memory T cell populations.
Protein Tyrosine Phosphatase Receptor Type C isoform RA functions as a key regulator of antigen receptor signaling in immune cells. By modulating phosphorylation states of Src family kinases and other signaling intermediates, CD45RA plays a critical role in setting activation thresholds for T cell receptor signaling. A short functional summary is that CD45RA fine tunes immune cell responsiveness and maintains proper signaling balance during early T cell activation and immune surveillance.
CD45RA is highly expressed on naive CD4 positive and CD8 positive T cells, while memory T cells typically downregulate CD45RA and express alternative CD45 isoforms. This differential expression pattern makes CD45RA antibody reagents essential tools in immunology research, particularly in studies of T cell development, immune aging, vaccine responses, and immune reconstitution. CD45RA is also expressed on subsets of B cells and other leukocytes, contributing to its broader relevance in leukocyte biology and immune profiling.
At the structural level, CD45RA contains a large extracellular domain, a single transmembrane region, and a cytoplasmic tail with dual phosphatase domains, one of which is catalytically active. These domains enable CD45RA to directly regulate intracellular signaling cascades downstream of antigen receptors. CD45RA antibody tools are commonly applied to study surface expression, cell subset distribution, and signaling regulation in immune cells under both physiological and pathological conditions.
From a disease relevance perspective, altered CD45RA expression has been associated with immune dysregulation, chronic infection, autoimmunity, and immune senescence. Changes in the balance between CD45RA positive naive T cells and memory T cell populations are frequently assessed in clinical and translational research settings. Clone 158-4D3 is designed to recognize CD45RA and support consistent detection of this isoform in research applications. CD45RA antibodies from NSJ Bioreagents are supplied for research use to support studies in immunophenotyping, immune monitoring, and cellular signaling.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RA antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Stimulated human leukocytes were used as the immunogen for this CD45RA antibody.
Store the CD45RA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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