CD45 Antibody SPM569 + SPM570 | V2402 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2402-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	559
Formulation
	V2402-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	259
Formulation
	V2402SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

CD45 Antibody SPM569 + SPM570 Tonsil IHC. Immunohistochemistry test of FFPE human tonsil probed with CD45 antibody cocktail.

CD45 Antibody SPM569 + SPM570 WB. Western blot testing of human spleen lysate with CD45 antibody cocktail (clones SPM569 + SPM570). Expected molecular weight: 147-220 kDa depending on glycosylation level.

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CD45 Antibody SPM569 + SPM570 Tonsil IHC. Immunohistochemistry test of FFPE human tonsil probed with CD45 antibody cocktail.

CD45 Antibody SPM569 + SPM570 WB. Western blot testing of human spleen lysate with CD45 antibody cocktail (clones SPM569 + SPM570). Expected molecular weight: 147-220 kDa depending on glycosylation level.

Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	SPM569 + SPM570
Purity	Protein G affinity chromatography
UniProt	P08575
Localization	Cell surface and cytoplasmic
Applications	Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This CD45 Antibody SPM569 + SPM570 is available for research use only.
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Description

CD45 Antibody SPM569 + SPM570 detects CD45, also referred to as CD45R and PTPRC (Protein tyrosine phosphatase receptor type C), identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Along with other members of the PTP family, it regulates a number of cellular processes including cell differentiation, growth and mitotic cycle, and is an essential regulator of B- and T-cell antigen receptor-mediated activation.

Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. CD45RA contains the A exon and is a naive T-cell marker which may help prevent autoimmune disease. CD45RB contains B and stains most leukemias and lymphomas. CD45RC contains C and stains thymocytes, monocytes and dendritic cells. CD45RO doesn't contain A, B or C and is a marker of activated T-cells that can be used to classify and diagnose and classify lymphomas. This antibody cocktail will bind to all CD45 isoforms. The variation in these isoforms is localized to the extracellular domain, with the intracellular domain being conserved. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

CD45 is encoded by PTPRC and functions as a key regulator of leukocyte signaling and hematopoietic cell biology; additional reagents targeting this protein are available on the CD45 Antibody page.

Application Notes

Due to variation in protocol and secondary antibody used, the CD45 Antibody SPM569 + SPM570 may need to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.