CD45RA Antibody PTPRC/1148 / Mouse Monoclonal [clone PTPRC/1148] (V2405)

Description

CD45RA Antibody PTPRC/1148 detectgs CD45RA, an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

CD45RA is an alternatively spliced form of PTPRC widely studied in lymphocyte differentiation and immune cell phenotyping; learn more about the target and related reagents on our CD45RA Antibody page.

Application Notes

Due to variation in protocol and secondary antibody used, the CD45RA Antibody PTPRC/1148 may need to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human T lymphocytes were used as immunogen for this CD45RA antibody.

Storage

The CD45RA antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.