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Home >> Antibodies >> Anti-CD45RA Antibody

Anti-CD45RA Antibody [clone CDLA45RA-1] (V7071)

  Catalog No Formulation Size Price (USD)  
Image V7071-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V7071-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V7071SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V7071IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of FFPE human tonsil and anti-CD45RA antibody (clone CDLA45RA-1). HIER: boil sections in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
Flow cytometry testing of PFA-fixed human Jurkat cells with CD45RA antibody (clone CDLA45RA-1); Red=isotype control, Blue= CD45RA antibody.
Western blot testing of human spleen lysate with CD45RA antibody (clone CDLA45RA-1). Expected molecular weight: 147-220 kDa depending on glycosylation level.
Immunofluorescence staining of PFA-fixed human Jurkat cells with CD45RA antibody (clone CDLA45RA-1, green) and Reddot nuclear stain (red).
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name CDLA45RA-1
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P08575
Localization Cell surface
Applications Western blot : 1-2ug/ml
Flow cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2 ug/ml
Immunohistology (FFPE) : 0.5-1.0ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This anti-CD45RA antibody is available for research use only.
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Description

CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells.

Application Notes

Titering of the anti-CD45RA antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as immunogen for this anti-CD45RA antibody.

Storage

The anti-CD45RA antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

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