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banner Search results for UBB

K27-linkage Specific Ubiquitin Antibody / UBB / UBC (clone 31U80)

Catalog No : FY13127
Applications : WB, IHC, ICC, IF
Reactivity : Human, Mouse, Rat
Format : Liquid
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Western blot analysis of K27-linkage Specific Ubiquitin expression in human HeLa cell lysate using K27-linkage Specific Ubiquitin antibody. A strong band near ~20 kDa in HeLa lysate is detected, corresponding to K27-linked di-ubiquitin. Although the UBB and UBC precursors have predicted masses of ~26 kDa and ~77 kDa, they are rapidly processed into monomeric and short polyubiquitin chains in cells; K27-linked Ub2 species are therefore the predominant form detected under standard conditions.
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Ubiquitin Antibody (clone rFPM1)

Catalog No : V6015
Applications : IHC
Reactivity : Human
Format : Purified
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Immunohistochemistry analysis of UBB/Ubiquitin antibody in human brain tissue (clone rFPM1). FFPE human brain sections demonstrate scattered HRP-DAB brown staining within neuronal cell bodies and glial cells, with a predominantly cytoplasmic punctate pattern and minimal nuclear signal. The staining appears granular and localized within the cytoplasm of scattered neural cells against a lightly stained neuropil background, consistent with ubiquitinated protein aggregates and intracellular ubiquitin pools. Surrounding parenchyma shows low diffuse background staining. The inset image shows the PBS secondary-only negative control, confirming absence of specific staining in the control section. Heat induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 minutes at 95C followed by cooling at room temperature prior to primary antibody incubation.
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Beta Tubulin Antibody / TUBB (clone BI-20)

Catalog No : RQ5179
Applications : IHC-P, IF/ICC, WB
Reactivity : Human, Mouse, Rat
Format : Purified
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IF/ICC staining of mouse NIH3T3 cells with Beta Tubulin antibody (red), PCNA antibody (green) and DAPI nuclear stain (blue).
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Beta Tubulin Antibody / TUBB (clone 5E4.)

Catalog No : RQ6081
Applications : WB, IF, FACS
Reactivity : Human, Mouse, Rat
Format : Purified
Immunofluorescent staining of FFPE human A431 cells with Tubulin Beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Beta Tubulin Antibody / TUBB (clone TUBB/4470)

Catalog No : V4450
Applications : IHC-P
Reactivity : Human
Format : Purified
IHC staining of FFPE prostate tissue with Beta Tubulin antibody (clone TUBB/4470). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Beta Tubulin Antibody / TUBB

Catalog No : F54148
Applications : WB
Reactivity : Mouse
Pred. Reactivity :Bovine, C. elegans, Chicken, Drosophila, Hamster, Human, Primate, Rat, Xenopus
Format : Antigen affinity purified
Western blot testing of mouse brain lysate with Beta Tubulin antibody at 1:2000. Predicted molecular weight: 50-55 kDa.
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Ubiquitin Antibody (clone UBB/3143R)

Catalog No : V8150
Applications : IHC-P, WB
Reactivity : Human
Format : Purified
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Western blot testing of human Raji cell lysate with Ubiquitin antibody (clone UBB/3143R). Expected molecular weight ~9 kDa.
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Ubiquitin Antibody (clone rUBB/9476)

Catalog No : V5804
Applications : IHC-P, WB
Reactivity : Human
Format : Purified
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Immunohistochemistry analysis of Ubiquitin antibody in human brain tissue (clone rUBB/9476). FFPE human brain sections demonstrate scattered HRP-DAB brown staining within neuronal cell bodies and glial cells, with predominantly cytoplasmic signal and occasional nuclear staining consistent with ubiquitin localization in both cytoplasmic and nuclear protein pools. The staining pattern reflects the widespread distribution of ubiquitinated proteins in neural tissue. Background staining is minimal, and surrounding neuropil shows light diffuse signal. The inset image shows the PBS secondary-only negative control, confirming absence of specific staining in the control section. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to primary antibody incubation.
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Ubiquitin Antibody (clone UBB/1748)

Catalog No : V3933
Applications : FACS, WB, IF, IHC-P
Reactivity : Human
Format : Purified
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Western blot testing of human Raji cell lysate with Ubiquitin/UBB antibody (clone UBB/1748). Expected molecular weight ~9 kDa.
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Ubiquitin Antibody (clone UBB/2122)

Catalog No : V3934
Applications : ELISA, WB, IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/2122). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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