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Home >> Antibodies >> Anti-Ubiquitin Antibody

Anti-Ubiquitin Antibody (F42055)

  Catalog No Formulation Size Price (USD)  
Image F42055-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F42055-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC staining of FFPE human breast carcinoma with anti-Ubiquitin antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney tissue with anti-Ubiquitin antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human 293, 2) human HeLa, 3) human HepG2, 4) human A549, 5) mouse NIH 3T3, 6) rat C2C12 and 7) rat C6 cell lysate with anti-Ubiquitin antibody. Predicted molecular weight ~8 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P0CG48
Localization Cytoplasmic, nuclear
Applications Western blot : 1:1000-1:2000
Immunohistochemistry (FFPE) : 1:100-1:500
Limitations This anti-Ubiquitin antibody is available for research use only.
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Description

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. It is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the anti-Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1-32 from the human protein was used as the immunogen for this anti-Ubiquitin antibody.

Storage

Can be stored at 4oC for one month. For long-term, aliquot the anti-Ubiquitin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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