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banner Search results for TTL

TTL Antibody / Tubulin-tyrosine ligase

Catalog No : FY13081
Applications : WB, ELISA
Reactivity : Mouse, Rat
Format : Lyophilized
Western blot analysis of TTL using anti-TTL antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTL antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A dominant band is detected at ~43 kDa, consistent with the predicted size of TTL. Two faint bands flanking the main band are observed and likely reflect differential phosphorylation (slower) and/or limited proteolysis (faster). Additional weak bands appear as a ~55 kDa doublet and a ~100 kDa doublet, compatible with low-abundance conjugated/complexed TTL species that are more apparent in neuronal tissue.
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TTLL1 Antibody / Tubulin-tyrosine ligase-like protein 1

Catalog No : FY12437
Applications : WB, IHC, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunohistochemical staining of TTLL1 using anti-TTLL1 antibody. TTLL1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TTLL1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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TTLL2 Antibody / Tubulin-tyrosine ligase-like protein 2

Catalog No : RQ7614
Applications : WB, IHC-P, IF, Direct ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human liver cancer with TTLL2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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TTLL3 Antibody / Tubulin Tyrosine Ligase Like 3

Catalog No : RQ7796
Applications : WB, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
Western blot testing of human 1) 293T, 2) HeLa and 3) Jurkat cell lysate with TTLL3 antibody. Predicted molecular weight ~87 kDa.
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TTLL4 Antibody

Catalog No : R33637
Applications : IHC, ELISA (peptide)
Reactivity : Human
Pred. Reactivity :Cow, Mouse, Rat
Format : Antigen affinity purified
IHC testing of FFPE human bronchus with TTLL4 antibody at 5ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
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TTLL4 Antibody

Catalog No : R35779
Applications : WB, ELISA (peptide)
Reactivity : Human, Mouse, Rat, Dog
Pred. Reactivity :Cow
Format : Antigen affinity purified
Western blot testing of MDCK lysate with TTLL4 antibody at 1ug/ml. Predicted molecular weight: ~133kDa.
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TTLL5 Antibody / Tubulin tyrosine ligase-like protein 5

Catalog No : FY12732
Applications : WB, ELISA
Reactivity : Human
Format : Lyophilized
Western blot analysis of TTLL5 using anti-TTLL5 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL5 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A predominant band is observed just above the 100 kDa marker, consistent with reported TTLL5 isoforms and known electrophoretic migration behavior of this protein (predicted ~144 kDa).
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TTLL9 Antibody / Tubulin tyrosine ligase-like protein 9

Catalog No : FY12070
Applications : WB, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of TTLL9 using anti-TTLL9 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human REH whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL9 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for TTLL9 at approximately 51 kDa. The expected band size for TTLL9 is at 51 kDa.
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TTLL12 Antibody / Tubulin Tyrosine Ligase Like 12

Catalog No : RQ7797
Applications : WB, FACS, IHC-P, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
IHC staining of FFPE human colorectal adenocarcinoma tissue with TTLL12 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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