TTLL10 Antibody / Inactive polyglycylase TTLL10 Antibody (FY13549)

TTLL10 Antibody by IF in human fallopian tube. Immunofluorescence analysis using Inactive polyglycylase TTLL10 Antibody shows red staining along the folded epithelial lining of human fallopian tube tissue, with blue DAPI nuclear counterstaining outlining the mucosal architecture. The merged image demonstrates TTLL10-associated fluorescence concentrated in epithelial regions bordering the luminal spaces. Paraffin-embedded sections underwent heat-mediated antigen retrieval in EDTA buffer at pH 8.0 and blocking with 10% goat serum. Primary antibody was incubated at 5 ug/mL overnight at 4oC, followed by a Cy3-conjugated anti-rabbit secondary antibody at 1:500.

TTLL10 Antibody by IHC in human fallopian tube. Immunohistochemistry of paraffin-embedded human fallopian tube using Inactive polyglycylase TTLL10 Antibody demonstrates brown staining along the epithelial lining of folded mucosal structures, with blue hematoxylin counterstaining identifying cell nuclei. The strongest immunoreactivity is associated with the epithelial compartment rather than the surrounding stromal tissue. Heat-mediated antigen retrieval was performed in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with 2 ug/mL primary antibody overnight at 4oC and developed using an HRP-conjugated secondary antibody and DAB chromogen.

TTLL10 Antibody by WB in human cell lines. Western blot analysis using Inactive polyglycylase TTLL10 Antibody detects a principal band near 75 kDa, consistent with the expected molecular weight of TTLL10. Lanes 1-4 contain HEL, HaCaT, MCF-7 and PC-3 whole-cell lysates, respectively. Reduced lysates containing 30 ug protein per lane were resolved by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, membranes were incubated with primary antibody at 0.5 ug/mL overnight at 4oC. HRP-conjugated anti-rabbit secondary antibody and chemiluminescent substrate were used for detection. The target-associated band is visible across the four examined human cell lines.

TTLL10 Antibody by FACS in HEL cells. Flow cytometry analysis using Inactive polyglycylase TTLL10 Antibody shows a blue antibody-stained HEL cell fluorescence distribution shifted to higher intensity than the green isotype control and red unstained control. Cells were fixed with 4% paraformaldehyde, permeabilized and blocked with normal goat serum before incubation with 1 ug primary antibody per 1 x 10^6 cells for 30 minutes at 20oC. Fluorescent anti-rabbit secondary antibody was applied for 30 minutes. The overlay histogram documents intracellular TTLL10-associated staining compared with the matched control distributions.