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banner Search results for TIGAR

TIGAR Antibody / TP53-induced glycolysis and apoptosis regulator

Catalog No : FY12776
Applications : WB, IHC, ICC/IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunohistochemical staining of TIGAR using anti-TIGAR antibody. TIGAR was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TIGAR antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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TIGAR Antibody / TP53-induced glycolysis and apoptosis regulator

Catalog No : FY12597
Applications : WB, IHC, IF, ELISA
Reactivity : Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of TIGAR using anti-TIGAR antibody (red). TIGAR was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-TIGAR antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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