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banner Search results for SYNC

SYNC Antibody / Syncoilin

Catalog No : FY12115
Applications : ELISA, IF, ICC, WB
Reactivity : Human
Format : Lyophilized
IF analysis of Syncoilin/SYNC using anti-SYNC antibody (red). SYNC was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SYNC antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Sync Antibody / Syncoilin

Catalog No : FY12164
Applications : ELISA, WB, FACS
Reactivity : Mouse, Rat
Format : Lyophilized
Western blot analysis of SYNC using anti-SYNC antibody. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat H9C2(2-1) whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNC antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for SYNC at approximately 72 kDa. The expected band size for SYNC is at 54 kDa but is also observed at 65-70 kDa.
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