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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
banner Search results for SON

SOS1 Antibody / Son of sevenless 1

Catalog No : FY12583
Applications : WB, IHC, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunohistochemical staining of SOS1 using anti-SOS1 antibody. SOS1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SOS1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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SON Antibody / Protein SON

Catalog No : FY12981
Applications : WB, IHC, IF, ICC/IF, FACS, ELISA
Reactivity : Human, Mouse
Format : Lyophilized
Immunofluorescent staining of SON using anti-SON antibody (green) and anti-Beta Tubulin antibody (red). SON was detected in an immunocytochemical section of Siha cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SON antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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