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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
banner Search results for NIT2
There are no search results with an exact match of NIT2. Related results are displayed below.

NIT2 Antibody / Nitrilase homolog 2

Catalog No : RQ8327
Applications : WB, IHC-P, FACS, Direct ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
IHC staining of FFPE human liver cancer tissue with NIT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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ORC2 Antibody / Origin recognition complex subunit 2

Catalog No : FY12600
Applications : WB, IHC, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of ORC2 using anti-ORC2 antibody. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORC2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot analysis of multiple cell and tissue lysates probed with anti-ORC2 shows a major band at ~74 kDa, slightly higher than the predicted ~68 kDa, consistent with phosphorylated ORC2. Additional weaker upper and lower bands represent differential phosphorylation states associated with cell-cycle regulation.
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NHP2 Antibody / H/ACA ribonucleoprotein complex subunit 2

Catalog No : RQ8225
Applications : WB, IF, FACS, IP, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human A549 cells with NHP2 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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RFC2 Antibody / Replication factor C subunit 2

Catalog No : RQ7817
Applications : WB, IF, FACS, Direct ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human PC-3 cells with RFC2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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PFDN2 Antibody / Prefoldin subunit 2

Catalog No : FY13323
Applications : WB, ICC/IF, FACS, ELISA
Reactivity : Human, Rat
Format : Lyophilized
Immunofluorescent staining of PFDN2 using anti-PFDN2 antibody (green) and anti-Beta Tubulin antibody (red). PFDN2 was detected in an immunocytochemical section of cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-PFDN2 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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QTRT2 Antibody / Queuine tRNA-ribosyltransferase subunit 2

Catalog No : FY12313
Applications : WB, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of QTRT2 using anti-QTRT2 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QTRT2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected molecular weight of QTRT2 is ~47 kDa.
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NCAPH Antibody / Condensin complex subunit 2

Catalog No : RQ8201
Applications : WB, IF, FACS, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human A549 cells with NCAPH antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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NCAPH Antibody / Condensin complex subunit 2

Catalog No : R36151
Applications : WB, ELISA (peptide)
Reactivity : Human
Format : Antigen affinity purified
Western blot testing of K562 cell lysate with NCAPH antibody at 0.01ug/ml. Predicted molecular weight: ~83kDa, routinely observed at 90~97kDa.
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