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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
banner Search results for NCL

NCL Antibody / Nucleolin (clone 30N86)

Catalog No : FY12161
Applications : WB, IHC, ICC, IF
Reactivity : Human, Mouse, Rat
Format : Liquid
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Western blot analysis of Nucleolin expression in K562 cell lysate using NCL antibody.
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NCL Antibody / Nucleolin (clone MSVA-623R)

Catalog No : V6101
Applications : IHC
Reactivity : Human
Format : Purified
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Nucleolin Rabbit Recombinant Monoclonal Antibody (MSVA-623R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas. <br><br>Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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NCLN Antibody / Nicalin

Catalog No : FY12359
Applications : WB, IHC, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunohistochemical staining of NCLN using anti-NCLN antibody. NCLN was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-NCLN antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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Nucleolin Antibody (clone NCL/7014R)

Catalog No : V8771
Applications : FACS, IF, WB, IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human salivary gland with Nucleolin antibody (clone NCL/7014R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Nucleolin Antibody (clone NCL/8695R)

Catalog No : V4962
Applications : WB, IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human skin tissue with Nucleolin antibody (clone NCL/8695R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Nucleolin Antibody (clone NCL/8068R)

Catalog No : V4963
Applications : WB, IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human colon cancer tissue with Nucleolin antibody (clone NCL/8068R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Nucleolin Antibody (pT84) (clone 29N17)

Catalog No : RQ8681
Applications : WB
Reactivity : Human
Format : Purified
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Western blot testing of lysate from human K562 cells, untreated (1) and Calyculin A treated (2), with Phospho-Nucleolin antibody.
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Nucleolin Antibody (pT76) (clone 29N32)

Catalog No : RQ8749
Applications : WB
Reactivity : Human
Format : Purified
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Western blot testing of lysate from human 293T cells, untreated (1) and Lambda Phosphatase treated (2), with Phospho-Nucleolin antibody.
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Nucleolin Antibody (clone 29N05)

Catalog No : RQ8805
Applications : WB
Reactivity : Human
Format : Purified
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Western blot testing of human HeLa cell lysate with NCL antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.
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Nucleolin Antibody (clone NCL/902)

Catalog No : V2755
Applications : FACS, IF, WB, IHC-P
Reactivity : Human, Mouse, Rat
Format : Purified
Western blot of A431 cell lysate using Nucleolin antibody.
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