- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Nucleolin Antibody Rabbit Monoclonal NCL/8695R [clone NCL/8695R] (V4962)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V4962-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
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V4962-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4962SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | NCL/8695R |
| Purity | Protein A/G affinity |
| UniProt | P19338 |
| Localization | Nucleoli |
| Applications | Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This Nucleolin antibody is available for research use only. |
|
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Nucleolin (NCL) is a multifunctional nucleolar phosphoprotein that plays a critical role in ribosome biogenesis, ribosomal RNA transcription, and nucleolar organization. The protein is encoded by the NCL gene on chromosome 2q37 and is one of the most abundant nucleolar proteins in proliferating cells. Nucleolin participates in transcription of ribosomal RNA genes, processing of precursor rRNA transcripts, and assembly of ribonucleoprotein complexes required for ribosome formation. The Nucleolin Antibody Rabbit Monoclonal NCL/8695R is designed to detect nucleolin expression in research studies investigating nucleolar structure and regulation of ribosome production.
Nucleolin is primarily localized within the nucleus and nucleolus where it functions as a central regulator of ribosomal RNA synthesis and nucleolar architecture. In actively proliferating cells, nucleolin accumulates strongly within nucleoli and contributes to the organization of ribosomal transcription complexes. Because nucleolin expression correlates with cellular proliferation and metabolic activity, antibodies targeting NCL are frequently used to study nucleolar biology and cellular growth pathways.
Nucleolin contains several structural domains that enable its diverse biological functions. The N-terminal acidic domain interacts with chromatin and ribosomal RNA transcription machinery. Four RNA recognition motifs in the central region allow nucleolin to bind ribosomal RNA and other RNA molecules, while the glycine- and arginine-rich C-terminal domain mediates interactions with nucleic acids and nucleolar proteins. Through these domains nucleolin coordinates ribosomal RNA transcription, RNA processing, and ribonucleoprotein assembly, processes that are tightly linked to cell growth and protein synthesis.
NCL antibody reagents are commonly referenced in the literature using several established synonyms for the protein. NCL antibody, nucleolin antibody, C23 nucleolin antibody, and nucleolar protein nucleolin antibody all refer to the same nucleolar phosphoprotein historically described as nucleolin nucleolar phosphoprotein. The designation C23 originates from early nucleolar protein fractionation experiments that identified nucleolin as a prominent nucleolar phosphoprotein associated with ribosomal transcription complexes. These alternative names remain widely used in studies of nucleolar biology and ribosome synthesis.
Nucleolin expression is frequently elevated in rapidly dividing cells and many tumor types where increased ribosome production supports enhanced protein synthesis required for cellular proliferation. Because nucleolin displays a characteristic nuclear and nucleolar localization pattern, antibodies targeting this protein provide useful tools for examining nucleolin distribution and nucleolar function in cellular studies. The Nucleolin Antibody Rabbit Monoclonal NCL/8695R therefore supports research focused on nucleolin expression, nucleolar organization, and regulation of ribosome biogenesis.
Optimal dilution of the Nucleolin Antibody Rabbit Monoclonal NCL/8695R should be determined by the researcher.
Recombinant full-length human NCL protein was used as the immunogen for the rabbit monoclonal Nucleolin antibody.
Aliquot the Nucleolin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
NCL antibody, C23 nucleolin antibody, nucleolar protein nucleolin antibody, nucleolin nucleolar phosphoprotein antibody
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