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  • Tel: 858.663.9055
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banner Search results for MED4

MED4 Antibody / Mediator complex subunit 4

Catalog No : FY13308
Applications : WB, IHC, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunohistochemical staining of MED4 using anti-MED4 antibody. MED4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MED4 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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MED4 Antibody

Catalog No : R32972
Applications : WB, IHC-P, Direct ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Western blot testing of rat spleen lysate with MED4 antibody at 0.5ug/ml. Predicted molecular weight ~30 kDa, commonly observed at 30-36 kDa.
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MED4 Antibody

Catalog No : RQ5989
Applications : WB, IHC-P, IF, FACS, Direct ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
IHC staining of FFPE human rectal cancer with MED4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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SUPT6H Antibody / SPT6 / Transcription elongation factor SPT6

Catalog No : FY13391
Applications : WB, ICC, IF, FACS, ELISA
Reactivity : Human, Monkey, Mouse, Rat
Format : Lyophilized
Western blot analysis of SUPT6H using anti-SPT6 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: monkey COS-7 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUPT6H antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substratewith Tanon 5200 system. A strong band was detected at approximately 250 kDa, which is higher than the predicted 199 kDa and consistent with the known slow-migrating behavior of SUPT6H caused by its acidic, low-complexity domains and extensive phosphorylation.
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