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banner Search results for HSPA4

HSPA4 Antibody / Heat shock protein family A member 4

Catalog No : FY13366
Applications : WB, IHC, ICC, IF, FACS
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of HSPA4 using anti-HSPA4 antibody. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA4 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A dominant band is observed at approximately 105-110 kDa, which is the expected migration pattern for HSPA4. Although the theoretical molecular weight of HSPA4 is ~94 kDa, members of the Hsp110 family, including HSPA4, are well known to migrate 10-20 kDa heavier due to acidic domains, extended disordered regions, and phosphorylation, which reduce SDS binding and cause anomalous gel mobility.
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HSPA4L Antibody / Heat shock 70 kDa protein 4L

Catalog No : RQ8806
Applications : WB, IF, FACS, IHC-P, ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human A549 cells with HSPA4L antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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HSPA4L Antibody / Heat shock 70 kDa protein 4L

Catalog No : R36492
Applications : IHC-P, ELISA (peptide)
Reactivity : Human
Format : Antigen affinity purified
IHC testing of FFPE human testis tissue with HSPA4L antibody at 5ug/ml. HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
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