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banner Search results for HBZ

HBZ Antibody / Hemoglobin zeta

Catalog No : FY12297
Applications : WB, ELISA
Reactivity : Mouse, Rat
Format : Lyophilized
Western blot analysis of HBZ using anti-HBZ antibody. Lane 1: rat bone tissue lysates, Lane 2: mouse bone tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBZ antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Expected molecular weight of Hemoglobin subunit zeta (HBZ) ~16 kDa (142 aa). On SDS-PAGE/Western blot, HBZ typically migrates at ~13–14 kDa due to its compact globin structure and atypical SDS binding. The observed band just below the 15 kDa marker is consistent with published reports for zeta-globin.
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HBAE3 Antibody / Zebrafish

Catalog No : RZ1388
Applications : WB, IHC
Reactivity : Zebrafish
Format : Antigen affinity purified
Zebrafish HBAE3 Antibody Erythrocyte IHC. Immunohistochemical staining of paraffin-embedded zebrafish erythrocytes using Zebrafish HBAE3 Antibody / Hemoglobin Alpha Embryonic 3 Antibody demonstrates strong HRP-DAB brown staining within clustered erythroid cells. The staining pattern is consistent with expression of HBAE3, an embryonic alpha-globin protein that contributes to hemoglobin complex formation and oxygen transport during early vertebrate development. HBAE3 is associated with hematopoietic tissues, developing blood cell populations, and the embryonic vasculature, where it supports oxygen delivery to rapidly growing tissues. The observed erythrocyte-associated staining is consistent with the established role of HBAE3 in embryonic hematopoiesis, erythroid differentiation, and developmental oxygen transport. Heat-mediated antigen retrieval was performed in EDTA buffer, followed by overnight incubation with 2 µg/ml primary antibody and HRP-DAB visualization.
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